多肉植物抖抖玉露组培快繁技术研究  

Study on Tissue Culture and Rapid Propagation of Haworthia cooperi

作　　者：周陆平 孔雪迪 陈建中[1] 田传玉 梁桂平 杨帆[1] ZHOU Luping;KONG Xuedi;CHEN Jianzhong;TIAN Chuanyu;LIANG Guiping;YANG Fan(School of Life Sciences,Huzhou University,Huzhou 313000,China)

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《湖州师范学院学报》2021年第2期39-42,共4页Journal of Huzhou University

基　　金：浙江省自然科学基金项目(LY16C020003);国家级大学生创新创业训练计划项目(201810347015)。

摘　　要：以抖抖玉露(Haworthia cooperi)试管苗为试验材料,研究基本培养基、植物生长调节剂配比及其质量浓度水平变化对试管苗生长、增殖和产生不定根的影响.结果表明:抖抖玉露试管苗的最佳继代增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,培养60 d后,增殖系数可达5.1;试管苗的最佳生根诱导培养基为1/2MS+IBA 0.2 mg/L,平均产生的不定根数为3.1.该研究结果为建立有效的抖抖玉露组培苗工厂化生产体系提供了理论依据.Effects of basic culture medium,plant growth regulator ratio and concentration level on the growth,proliferation and adventitious root formation of Haworthia cooperi shoots were studied to provide a theoretical basis for establishing an effective industrialized production system of tissue culture shoots of Haworthia cooperi.The results showed that the best proliferation medium for Haworthia cooperi shoots was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L and the multiplication coefficient was 5.1 after 60 days of culture.The best rooting medium for Haworthia cooperi shoots was 1/2MS+IBA 0.2 mg/L and the average number of adventitious roots was 3.1.

关 键 词：抖抖玉露 组织培养 快速繁殖 

分 类 号：X503.23[环境科学与工程—环境工程]