鸭坦布苏病毒病-H9亚型禽流感二联灭活疫苗免疫佐剂筛选  

Screening of Duck Tembusu Virus-H9 Subtype Avian Influenza Virus Inactivated Vaccine Adjuvant

作　　者：田宇杰 嵇辛勤[1] 章丽娇 刘青涛[2] 韩凯凯[2] 杨婧[2] 刘宇卓[2] 赵冬敏[2] 刘娜[2] 王梦瑶 李银[2] TIAN Yu-jie;JI Xin-qing;ZHANG Li-jiao;LIU Qing-tao;HAN Kai-kai;YANG Jing;LIU Yu-zhuo;ZHAO Dong-min;LIU Na;WANG Meng-yao;LI Yin(College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Animal Disease Diagnosis and Immunology,Ministry of Agriculture/ National Research Center for Engineering and Technology of Veterinary Bio-products,Nanjing,Jiangsu 210014,China;Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏南京210014 [3]贵州省农业科学院畜牧兽医研究所,贵州贵阳550025

出　　处：《动物医学进展》2021年第5期26-29,共4页Progress In Veterinary Medicine

基　　金：水禽重要疫病免疫防控新技术研究(2017YFD0500800)。

摘　　要：为筛选出理想的鸭TMUV-AIV(H9亚型)二联灭活疫苗免疫佐剂,将灭活的坦布苏病毒液和禽流感病毒(H9亚型)尿囊液分别与白油佐剂、卡波姆佐剂、铝胶佐剂、蜂胶佐剂制成抗原含量相同的TMUV-AIV二联灭活疫苗。应用阻断ELISA方法和血凝抑制方法测定免疫鸭血清中坦布苏病毒和禽流感病毒抗体,比较不同佐剂疫苗对试验鸭的免疫效力。结果表明,以白油作为佐剂的灭活疫苗组免疫效果最好,抗体产生快、抗体水平优于其他各组,免疫2周后60%(6/10)血清样品HI抗体可达1∶8以上,80%血清样品呈TMUV抗体阳性,是制备鸭TMUV-AIV(H9亚型)二联灭活疫苗的理想佐剂。To identify the best adjuvant for the combined vaccine of Duck Tembusu virus(DTMUV)and H9 avian influenza virus(H9 subtype),vaccines were prepared with inactivated DTMUV JS804 and H9N2 AIV NJ01 using oil,Carbomer,aluninum hydroxide or propolis adjuvant in this study.Each vaccine formulation was evaluated for immunogenicity in ducks by comparing antibody levels of DTMUV and AIV(H9 subtype)in serum samples collected at different time points of post immunization.The results showed that oil adjuvant vaccine could induce a faster antibody-producing process and higher antibody levels than the others.And the HI titers of AIV(H9 subtype)in 60%(6/10)serum samples was above 1∶8,and the TMUV seroconversion rate was 80%(8/10)by 2 weeks post vaccination.This study confirmed that oil is the optimum adjuvant for the preparation of DTMUV-AIV(H9 subtype)combined vaccine.

关 键 词：坦布苏病毒 禽流感病毒(H9亚型) 佐剂筛选 灭活疫苗 

分 类 号：S852.4[农业科学—基础兽医学] S858.32[农业科学—兽医学]