hsa-miR-125a的靶基因预测及生物信息学分析  

Target Gene Prediction and Bioinformatics Analysis of hsa-miR-125a

作　　者：杨一秋 李兰 赵娜[1] 解继胜 YANG Yiqiu;LI Lan;ZHAO Na(Master of Immunology in Youjiang Medical College for Nationalities,Baise City,Guangxi 533000)

机构地区：[1]右江民族医学院免疫学专业在读硕士研究生,广西百色市533000 [2]右江民族医学院基础医学院组织胚胎学教研室

出　　处：《医学理论与实践》2021年第9期1444-1446,共3页The Journal of Medical Theory and Practice

基　　金：国家自然科学基金资助项目(81560362)。

摘　　要：目的:通过生物信息学分析方法预测hsa-miR-125a的靶基因并对靶基因进行GO、KEGG富集分析。方法:利用miRBase数据库分析hsa-miR-125a的物种保守性;利用TargetScan、miRTarBase、miRDB数据库预测hsa-miR-125a靶基因,再将预测的靶基因利用DAVID数据库进行基因本体(GO)功能富集分析及京都基因与基因组百科全书(KEGG)生物通路富集分析。结果:hsa-miR-125a预测的交集靶基因共有122个。GO富集分析结果显示,hsa-miR-125a的靶基因主要参与细胞对DNA损伤刺激的反应、蛋白质泛素化的正调控等生物过程(均P<0.01)。KEGG信号通路富集分析结果显示,hsa-miR-125a的靶基因显著富集于endocytosis signaling pathway、adherens junction signaling pathway、TGF-βsignaling pathway、HIF-1 signaling pathway(P<0.05)。结论:hsa-miR-125a的靶基因富集的信号通路与破骨细胞生成密切相关,尤其是TGF-βsignaling pathway。Objective:To predict the target genes of hsa-miR-125a by bioinformatics analysis and to analyze the enrichment of GO and KEGG.Methods:miRBase database was used to analyze the species conservatism of hsa-miR-125a;TargetScan,miRTarBase and miRDB databases were used for target gene prediction,and DAVID database was used to analyze gene ontology(GO)function enrichment and Kyoto Encyclopedia of genes and genomes(KEGG)bioaccumulation analysis.Results:There were 122 target genes predicted by hsa-miR-125a.Go analysis showed that the target gene of hsa-miR-125a was mainly involved in cell response to DNA damage and the positive regulation of protein ubiquitination(all P<0.01).KEGG signaling pathway analysis showed that the target genes of hsa-miR-125a were significantly enriched in endocytosis signaling pathway,adhesions junction signaling pathway,TGF-βsignaling pathway and HIF-1 signaling pathway(P<0.05).Conclusion:The signal pathway of hsa-miR-125a target gene enrichment is closely related to osteoclast formation,especially TGF-βsignaling pathway.

关 键 词：破骨细胞 hsa-miR-125a 靶基因 信号通路 生物信息学 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]