NF-κB和iNOS在ALT≥400U/L慢性乙型肝炎患者的表达及临床意义  

The Expression and Clinical Significance of NF-κB and iNOS in Patients with Chronic Hepatitis B(ALT≥400U/L)

作　　者：陈邦银 黄津 CHEN Bangyin;HUANG Jin(Department of Infection,Nanping First Hospital Affiliated to Fujian Medical University,Nanping City,Fujian Province 353000)

机构地区：[1]福建医科大学附属南平第一医院感染科,福建省南平市353000

出　　处：《医学理论与实践》2021年第9期1447-1449,共3页The Journal of Medical Theory and Practice

基　　金：福建省卫生计生委青年基金(2017-1-94)。

摘　　要：目的:研究慢性乙型肝炎患者外周血中核因子-κB(Nuclear factor-κB,NF-κB)、诱导型一氧化氮合成酶(inducible nitric oxide synthase,iNOS)的表达情况,分析二者的临床价值。方法:纳入2017年4月10日—2020年4月10日于我院住院的慢性乙型肝炎患者80例作为肝炎组,60例健康者作为对照组。采用RT-PCR法检测NF-κB mRNA和iNOS mRNA的表达;Western Blot法检测外周血中NF-κB、iNOS蛋白的表达;ELISA法检测血清中NO水平。结果:(1)RT-PCR法检测结果发现:肝炎组NF-κB mRNA和iNOS mRNA的表达水平均高于对照组(均P<0.05);(2)Western Blot法检测结果发现:肝炎组NF-κB和iNOS蛋白表达水平均高于对照组(均P<0.05);(3)肝炎组NO水平高于对照组(P<0.05)。结论:本研究发现CHB患者NF-κB、iNOS表达水平较健康组明显升高,且分泌大量炎症因子NO,在HBV感染的致炎过程中发挥重要作用。Objective:To study the expression of nuclear factor-κB(NF-κB)and inducible nitric oxide synthase(iNOS)in peripheral blood of patients with chronic hepatitis B,and analyze their clinical value.Methods:A total of 80 patients with chronic hepatitis B who were hospitalized in our hospital from April 10th 2017 to April 10th 2020 were included as the hepatitis group,and 60 healthy peoples were included as the control group.RT-PCR method was used to detect the expression of NF-κB mRNA and iNOS mRNA;Western Blot method was used to detect the expression of NF-κB and iNOS protein in peripheral blood;ELISA method was used to detect the level of NO in serum.Results:(1)The results of RT-PCR method showed that:the expression levels of NF-κB mRNA and iNOS mRNA in the hepatitis group were significantly higher than the control group(both P<0.05);(2)The Western Blot method showed that:the expression levels of NF-κB and iNOS protein in hepatitis group were significantly higher than control group(all P<0.05);(3)The NO level of hepatitis group was significantly higher than that of the control group(P<0.05).Conclusion:This study found that the expression levels of NF-κB and iNOS in CHB patients were significantly higher than those in the healthy group,and they secreted a large amount of inflammatory factor NO,which played an important role in the inflammation process of HBV infection.

关 键 词：乙肝 核因子-ΚB 诱导型一氧化氮合成酶 一氧化氮 

分 类 号：R512.62[医药卫生—内科学]