水杨酸处理对镉胁迫下孔雀草组织显微结构的影响  

Effects of Salicylic Acid Treatments on Tissue Microstructure of Tagetes patula L.Under Cadmium Stress

作　　者：刘玉婷 敖雯雯 廖海民[1] 陈琳[1] 韩淑梅[1] LIU Yuting;AO Wenwen;LIAO Haimin;CHEN Lin;HAN Shumei(College of Life Science,Guizhou University,Guizhou Guiyang 550025)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《生物化工》2021年第2期47-51,共5页Biological Chemical Engineering

基　　金：山地植物资源保护与种质创新省部共建教育部重点实验室开放课题(MOELP-201705);广东省应用植物学重点实验室开放课题(AB2018006)。

摘　　要：目的:探究水杨酸(SA)强化处理缓解镉胁迫对孔雀草各组织结构毒害的影响。方法:采用盆栽试验,人工模拟100 mg/kg镉污染土壤,选择系列浓度的水杨酸,每隔5 d对镉超富集植物孔雀草进行喷施处理,处理浓度分别为0.1 mmol/L、0.2 mmol/L、0.3 mmol/L、0.4 mmol/L,对应T1、T2、T3及T4组,结合生物显微石蜡切片技术观察各组孔雀草的根、茎、叶的组织结构变化。结果:镉胁迫下,孔雀草根、茎、叶各组织细胞都有不同程度的裂解和损伤,而水杨酸喷施处理能有效缓解该毒害作用,增加其镉胁迫耐受能力,各组耐受能力综合表现为T2>T3>T1>T4。结论:水杨酸处理是提升孔雀草对土壤镉污染修复能力的一个有力强化措施,为将孔雀草应用于土壤重金属镉污染治理提供了进一步的理论参考。Effects of Salicylic Acid(SA)treatments on tissue microstructure of Tagetes patula L.growing in simulated soil with Cd contamination(100 mg/kg)were investigated by pot experiment.Cd-hyperaccumulator Tagetes patula L.were sprayed every 5 d with a series of concentration SA treatments(0.1 mmol/L,0.2 mmol/L,0.3 mmol/L,0.4 mmol/L),corresponding to group T1,T2,T3 and T4.Combining paraffin section technology with digital biology microscope set to observe the microstructure variation of Tagetes patula L.including the roots,stems and leaves.The results showed that the tissue cells mentioned above were in different degrees of cleavage and damage under Cd Stress(100 mg/kg),and the toxicity of cadmium could be effectively alleviated after SA spraying treatment and the stress resistance was increased.And the Cd tolerance performance was presented as T2>T3>T1>T4,which meant that SA could be used as a powerful reinforcement measure for promoting the phytoremediation capability of Tagetes patula L.on Cd contaminated soil,and further theoretical reference was provided for Cd pollution management using Tagetes patula L..

关 键 词：孔雀草 土壤污染 镉胁迫 水杨酸 

分 类 号：X503.23[环境科学与工程—环境工程] X171.5