基于高效液相色谱法结合层次分析和正交设计法优选土茯苓醇提工艺  

Optimization of ethanol extraction of Smilacis Glabrae Rhizoma based on HPLC combined with the hierarchy analysis and orthogonal design

作　　者：黄诗淇 武正华 缪文清 周利娟 石文清 范国荣[2] HUANG Shi-qi;WU Zheng-hua;MIAO Wen-qing;ZHOU Li-juan;SHI Wen-qing;FAN Guorong(College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Nanning 530001;Department of Clinical Pharmacy,Shanghai General of Hospital,Shanghai Jiao Tong University,Shanghai 200080)

机构地区：[1]广西中医药大学药学院,南宁530001 [2]上海交通大学附属第一人民医院临床药学科,上海200080

出　　处：《中南药学》2021年第4期642-648,共7页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81973289);2018年度上海市临床重点专科建设项目(No.02.DY11.03.19.03)。

摘　　要：目的优选土茯苓中5种二氢黄酮醇类化合物的醇提工艺。方法利用HPLC法测定土茯苓中落新妇苷、新落新妇苷、异落新妇苷、新异落新妇苷和黄杞苷的含量。以上述5种化学成分的含量为评价指标,应用层次分析法确定各指标权重系数,再采用正交设计试验考察提取时间、固液比、乙醇浓度对工艺的影响。结果最佳提取工艺条件为土茯苓粉末以10倍量60%浓度的乙醇浸泡8 h,超声提取2次,每次提取30 min。结论层次分析法结合正交设计试验优化的提取工艺合理可行,为优化土茯苓中二氢黄酮醇类成分的生产工艺提供了参考依据。Objective To optimize the ethanol extraction of 5 kinds of dihydroflavonols from Smilacis Glabrae Rhizoma.Methods The content of astilbin,neoastilbin,isoastilbin,neoisoastilbin and engeletin in Smilacis Glabrae Rhizoma was determined by HPLC.The content of the 5 chemical components was used as evaluation indexes,hierarchy analysis and orthogonal design were used to investigate the influence of extraction time,solid-liquid ratio and the concentration of ethanol on the process.Results The optimal extraction conditions were as follows:the Smilacis Glabrae Rhizoma powder was soaked with 10-fold of 60%ethanol for 8 h,ultrasonic extraction twice,and each extraction lasting 30 min.Conclusion The extraction process optimized by hierarchy analysis combined with orthogonal design is reasonable and feasible,which provides a reference for optimizing the extraction of dihydroflavonols from Smilacis Glabrae Rhizoma.

关 键 词：土茯苓 二氢黄酮醇 提取工艺 层次分析法 正交设计 

分 类 号：R284[医药卫生—中药学]