慢性HIV感染者流式细胞仪检测外周血CD160的表达及其意义  

The Expression of CD160 in Peripheral Blood of Chronic HIV Infected Patients and Its Significance were Detected by Flow Cytometry

作　　者：郑怡娟 余雪平[1] 张华堂[1] 董明亮 郑明慧 李菊兰 苏智军[1] 苏密龙[3] ZHENG Yi-juan;YU Xue-ping;ZHANG Hua-tang;DONG Ming-liang;ZHENG Ming-hui;LI Ju-lan;SU Zhi-jun;SU Mi-long(The Department of Infectious Diseases,Quanzhou First Hospital Affiliated to Fujian Medical University,Fujian Quanzhou 362000;Quanzhou Hospital for Infectious Diseases,Fujian Quanzhou 362000;The Department of Clinical Laboratory,Quanzhou First Hospital Affiliated to Fujian Medical University,Fujian Quanzhou 362000)

机构地区：[1]福建医科大学附属泉州第一医院感染病科,福建泉州362000 [2]泉州市传染病院,福建泉州362000 [3]福建医科大学附属泉州第一医院检验科,福建泉州362000

出　　处：《中国医疗器械信息》2021年第8期22-23,172,共3页China Medical Device Information

基　　金：福建省自然科学基金(项目名称:HIV感染患者外周血CD57抗原及共抑制分子CD160的表达研究,项目编号:2017J01228);福建省自然科学基金面上项目(项目名称:BTLA调控lncRNA-NONHSAT178169.1/FOS信号途径介导HBV免疫逃逸的分子机制研究,项目编号:2019J01593);福建省科技创新联合资金项目(项目名称:B和T细胞弱减因子诱导HBV免疫耐受的分子机制研究,项目编号:2019Y9048);福建省卫健委中青年骨干人才培养项目(项目名称:BTLA/LncRNA/FOS轴在HBV免疫耐受中的作用机制研究,项目编号:2020GGA076);福建省卫生计生青年科研课题(项目名称:HBeAg通过滤泡辅助性T细胞诱导免疫耐受的机制研究,项目编号:2018-1-94);福建省卫生计生青年科研课题(项目名称:ICOS/ICOSL信号通路在HBV慢性感染中的表达及作用机制研究,项目编号:2018-1-95);泉州市高层次人才创新项目(项目名称:BTLA调控lncRNA-NONHSAT178169.1/FOS信号途径介导HBV免疫逃逸的分子机制研究,项目编号:2018C067R);泉州市科技项目(项目名称:ICOS分子在HBV慢性感染中的表达及作用机制研究,项目编号:2018Z069);泉州市科技项目(项目名称:Tfh细胞在慢性HBV感染中诱导免疫耐受的机制研究,项目编号:2018Z074)。

摘　　要：目的:探讨慢性HIV感染者外周血T和B淋巴细胞上CD160的表达特征及与疾病严重程度的相关性。方法:采用流式细胞仪检测81例慢性HIV感染者[33例长期无进展者(LTN)、25例典型进展者(TP)和23例艾滋病者(AIDS)]及35例健康对照者(NC)的T和B淋巴细胞上CD160的表达比例及平均荧光强度,分析其与疾病进展的相关性。结果:LTN和TP患者及NC组外周血CD3^(+)T、CD4^(+)T、CD8^(+)T及B淋巴细胞上CD160的表达比率和平均荧光强度均明显低于AIDS患者,差异具有统计学意义(P<0.05)。HIV感染者B细胞(r=-0.403,P=0.002)、CD3+T细胞(r=-0.572,P<0.001)、CD4^(+)T细胞(r=-0.564,P<0.001)及CD8^(+)T细胞(r=-0.600,P<0.001)上CD160分子表达量均与HIV RNA载量呈显著负相关。结论:AIDS患者中B和T细胞上CD160分子表达量明显增加,与HIV RNA水平呈负相关。Objective:To investigate the expression of CD160 in peripheral blood T and B lymphocytes of chronic HIV infected patients and its correlation with the severity of the disease.Methods:Flow cytometry was used to detect the expression ratio and mean fluorescence intensity of CD160 in T and B lymphocytes of 81 chronic HIV infected patients(33 long-term progression-free(LTN),25 typical progression-free(TP)and 23 AIDS patients)and 35 healthy controls(NC).The correlation between CD160 expression and disease progression was analyzed.Results:The expression ratio and mean fluorescence intensity of CD160 on peripheral blood lymphocytes of CD3^(+)T,CD4^(+)T,CD8^(+)T and B lymphocytes of LTN and TP patients and NC group were significantly lower than those of AIDS patients,with statistical significance(P<0.05).B cells(r=-0.403,P=0.002),CD3^(+)T cells(r=-0.572,P<0.001),CD4^(+)T cells(r=-0.564,P<0.001)and CD8^(+)T cells(r=-0.600,P<0.001)the expression of CD160 was negatively correlated with the HIV RNA load.Conclusion:The expression of CD160 in B and T cells was significantly increased in AIDS patients,and was negatively correlated with the level of HIV RNA.

关 键 词：HIV AIDS T细胞 B细胞 CD160 

分 类 号：R446.1[医药卫生—诊断学]