海藻酸钙/枸杞多糖凝胶微球对小鼠骨质疏松的作用研究  

Effect of calcium alginate/lycium barbarum polysaccharides gel microspheres on osteoporotic mice

作　　者：钟诚 孙国栋[2] 范全 杨东亮 梁伟乔 贾海升 李宇明 ZHONG Cheng;SUN Guodong;FAN Quan;YANG Dongliang;LIANG Weiqiao;JIA Haisheng;LI Yuming(Department of Orthopaedics, Jiangmen Hospital of Traditional Chinese Medicine Affiliated to Jinan University, Jiangmen 529000, China;Department of Orthopaedics, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China;Department of Orthopaedics, Jiangmen Hospital Affiliated to Sun Yat-sen University, Jiangmen 529000, Guangdong, China)

机构地区：[1]暨南大学附属江门中医院骨科,广东江门529000 [2]暨南大学附属第一医院骨科,广东广州510632 [3]中山大学附属江门医院骨科,广东江门529000

出　　处：《中国骨质疏松杂志》2021年第3期319-323,336,共6页Chinese Journal of Osteoporosis

基　　金：广东省中医药局重点科研项目(20173018)。

摘　　要：目的研究海藻酸钙(ALG-Ca)/枸杞多糖(LBPs)凝胶微球对小鼠骨质疏松的作用。方法采用静电液滴法制备ALG-Ca凝胶微球、ALG-Ca/LBPs凝胶微球,用扫描电镜观察微球结构。建立去卵巢小鼠骨质疏松模型,随机分为假手术组、模型组、单纯ALG-Ca微球组、单纯LBPs组、ALG-Ca/LBPs凝胶微球组,每组8只,每日灌胃1次,干预时间为12周,对各组小鼠股骨行HE染色观察骨组织形态变化,Micro-CT检测并经三维重建获得骨组织微观结构,对小鼠血清骨钙素(BGP)、血清碱性磷酸酶(BALP)、1型胶原羧基末端肽(CTX-1)进行检测。结果扫描电镜观察示凝胶微球呈均匀一致的微球形结构;OVX+LBPs、OVX+ALG-Ca/LBPs组的BGP、BALP、CTX-1均低于OVX+PBS组(P<0.05);OVX+LBPs、OVX+ALG-Ca/LBPs组骨体积分数(BV/TV)、骨小梁数目(Tb.N)及骨小梁厚度(Tb.Th)均高于OVX+PBS组(P<0.05),骨小梁间隙(Tb.Sp)小于OVX+PBS组(P<0.05),以OVX+ALG-Ca/LBPs组为最佳(P<0.01)。结论ALG-Ca凝胶微球是LBPs治疗骨质疏松的良好药物缓释载体;LBPs为天然植物成分,通过LBPs/ALG-Ca凝胶微球可以改善骨质疏松小鼠的骨代谢及骨微结构。Objective To studyt the effect of calcium alginate(ALG-Ca)/lycium barbarum polysaccharides(LBPs)gel microspheres on osteoporotic mice.Methods ALG-Ca gel microspheres and ALG-Ca/LBPs gel microspheres were prepared with electrostatic droplet method.The microsphere structure was observed with scanning electron microscopy.The osteoporosis model of ovariectomized mice was established.The mice were randomly divided into sham operation group,injury only group,ALG-Ca microsphere only group,LBPs only group,and ALG-Ca/LBPs gel microsphere group,with 8 mice in each group.The mice were intragastrically administered once a day for 12 weeks.The femoral bones of each group were stained with HE staining to observe the morphological changes of bone tissue.The microstructure of bone tissue was obtained with micro-CT and three-dimensional reconstruction.Serum osteocalcin(BGP),serum alkaline phosphatase(ALP),and C-terminal cross-linking telopeptides of type I collagen(CTX-1)were detected in mice.Results Scanning electron microscopy result showed that the gel microspheres showed a uniform microsphere structure.Levels of BGP,BALP,and CTX-1 in OVX+LBPs and OVX+ALG-Ca/LBPs groups were lower than those in OVX+PBS group(P<0.05).Bone volume fraction(BV/TV),trabecular number(Tb.N),and trabecular thickness(Tb.Th)were significantly higher than those in OVX+PBS group(P<0.05),and trabecular space(Tb.Sp)was lower than that in OVX+PBS group(P<0.05).The parameters in OVX+PBS group(P<0.05)and OVX+ALG-Ca/LBPs group were the best.Conclusion ALG-Ca gel microspheres are good drug delivery carriers for LBPs in the treatment of osteoporosis.LBPs are natural botanical components,which can improve bone metabolism and bone microstructure in osteoporotic mice through LBPs/ALG-Ca gel microspheres.

关 键 词：骨质疏松 枸杞多糖 海藻酸钙 去卵巢小鼠 

分 类 号：R681[医药卫生—骨科学]