新疆双峰驼乳清对棕榈酸损伤MIN6细胞的保护作用  

Protective Effect of Whey Protein from Xinjiang Bactrian Camel on Palmitic Acid Injury of MIN6 Cells

作　　者：侯志梅[1] 刘宸 梁敏[1] 丁雪 王桂玲[1] 冯鑫欢 杨洁[2] HOU Zhimei;LIU Chen;LIANG Min;DING Xue;WANG Guiling;FENG Xinhuan;YANG Jie(The Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China;College of life science and technology,Xinjiang University,Urumqi 830046,China;People's Hospital of Miquan County,Miquan 831400,China)

机构地区：[1]新疆医科大学第二附属医院,乌鲁木齐830063 [2]新疆大学生命科学与技术学院,乌鲁木齐830046 [3]米东县人民医院,新疆米泉831400

出　　处：《中国乳品工业》2021年第4期4-8,18,共6页China Dairy Industry

基　　金：新疆维吾尔自治区自然科学基金面上项目(2017D01C250)。

摘　　要：探讨驼乳清对小鼠胰岛β细胞株(MIN6)细胞损伤的保护作用以及信号通路的影响。通过棕榈酸构建MIN6细胞损伤模型,驼乳清处理后观察细胞活性,通过台盼蓝观察细胞形态,Hoechst染色观测细胞凋亡情况。通过Westernblot和QPCR检测MIN6细胞信号通路的表达。结果表明驼乳清能够有效提高MIN6损伤后的细胞活性,且驼乳清有较好的保护作用,上调了PI3K、AKT基因和蛋白水平的表达。结论为驼乳清能够有效改善胰岛细胞的损伤,抑制凋亡,这可能与驼乳激活了PI3K-AKT信号通路有关。This research was conducted to investigate the protective effect of camel whey on mouse isletβ-cell line(MIN6)cell damage and the effect of signal pathway.MIN6 cell injury model was established by palmitic acid.The cell viability was observed after camel whey treatment.The cell morphology was observed by trypan blue and apoptosis was observed by Hoechst staining.Western blot and qPCR were used to detect the expression of signal pathway in MIN6 cells.The results showed that camel whey could effectively improve the cell viability after MIN6 injury,and camel whey had a good protective effect,up regulating the expression of PI3K and Akt genes and proteins.Conclusion:Camel whey can effectively improve the damage of islet cells and inhibit apoptosis,which may be related to the activation of PI3K-Akt signaling pathway.

关 键 词：小鼠胰岛Β细胞 驼乳清 细胞损伤 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]