基于Co_(3)O_(4)纳米粒子的“串联酶”活性检测葡萄糖的表面增强拉曼光谱策略  

Surface-Enhanced Raman Scattering Strategy for Glucose Detection Based on“Tandem Enzyme”Activity of Co_(3)O_(4)Nanoparticles

作　　者：夏学敏 张霞[1] 翁仪瑾 张垒 唐如意 XIA Xue-Min;ZHANG Xia;WENG Yi-Jin;ZHANG Lei;TANG Ru-Yi(Material Engineering College,Shanghai University of Engineering Science,Shanghai 201620,China)

机构地区：[1]上海工程技术大学材料工程学院,上海201620

出　　处：《无机化学学报》2021年第5期809-816,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金青年基金(No.61404082)资助。

摘　　要：采用一种简单的湿化学法合成Co_(3)O_(4)纳米粒子(NPs),并将其作为一种"串联酶"(同时具有类过氧化物酶和类葡萄糖氧化酶活性)用于过氧化氢(H_(2)O_(2))和葡萄糖的表面增强拉曼散射(SERS)光谱检测。作为一种灵敏的SERS底物,在pH=4.0的NaAc缓冲液条件下,Co_(3)O_(4)NPs可以催化葡萄糖和O_(2)生成葡萄糖酸和H_(2)O_(2)。然后H_(2)O_(2)可以氧化3,3′,5,5′-四甲基联苯胺(TMB),形成蓝色氧化产物氧化TMB(oxTMB),其在1188、1330、1610 cm^(-1)处表现出强烈的SERS信号。因此,我们开发了一种新的SERS策略来分析葡萄糖,检测限为1×10^(-10)mol·L^(-1),表明Co_(3)O_(4)NPs具有生物传感器、免疫分析和医学研究的潜力。A simple wet-chemical method was used to synthesize Co_(3)O_(4)nanoparticles(NPs)as a"tandem enzyme"(with peroxidase-like and glucose-oxidase-like activities)for unlabeled hydrogen peroxide(H_(2)O_(2))and surfaceenhanced Raman scattering(SERS)for glucose.As a sensitive SERS substrate,Co_(3)O_(4)NPs can catalyze the production of gluconic acid and H_(2)O_(2)from glucose and O_(2)under the condition of pH=4.0 NaAc buffer.And then H_(2)O_(2)can oxidize 3,3′,5,5′-tetramethylbenzidine(TMB)to form the blue oxidation product oxidized TMB(oxTMB),which showed a strong SERS signal at 1188,1330,1610 cm-1.Therefore,we developed a new SERS strategy to analyze glucose with a detection limit of 1×10^(-10)mol·L^(-1),indicating that Co_(3)O_(4)NPs have the potential for biosensors,immu-noassays,and medical research.

关 键 词：表面增强拉曼散射 Co_(3)O_(4)纳米粒子 3 3′ 5 5′-四甲基联苯胺 催化 

分 类 号：O643.132[理学—物理化学] O657.37[理学—化学]