黄芩治疗高血压的网络药理学研究  

Study on the network pharmacology of Scutellariae Radix in the treatment of hypertension

作　　者：董明月 黄艳[2] 周立华[2] DONG Mingyue;HUANG Yan;ZHOU Lihua(The Third Clinical Medical College,He’nan University of Chinese Medicine,He’nan Province,Zhengzhou450000,China;Traditional Chinese Medicine Clinic,the Third Affiliated Hospital of He’nan University of Chinese Medicine,He’nan Province,Zhengzhou450000,China)

机构地区：[1]河南中医药大学第三临床医学院,河南郑州450000 [2]河南中医药大学第三附属医院国医馆,河南郑州450000

出　　处：《中国医药导报》2021年第12期15-19,F0003,共6页China Medical Herald

基　　金：国家中医药管理局全国名老中医药专家传承工作室建设项目-王国斌全国名老中医药专家传承工作室。

摘　　要：目的通过网络药理学分析黄芩治疗高血压的潜在作用机制。方法运用中药系统药理学数据库与分析平台(TCMSP)筛选黄芩的化学成分和作用靶点,利用GeneCards、OMIM、TTD、DrugBank数据库筛选出高血压的疾病靶点,运用Cytoscape 3.7.2软件构建黄芩治疗高血压的“有效成分-疾病靶点”网络,借助String数据库构建蛋白质-蛋白质相互作用(PPI)网络,最后利用R语言对作用靶点进行GO注释分析和KEGG通路分析。结果共得到86个黄芩与高血压相互作用靶点,其中CCND1、CDKN1A、ESR1等为核心基因。GO功能富集主要包括细胞对化学应激的反应、氧化应激反应、肾上腺素能受体活性等。KEGG通路中,卡波西肉瘤相关疱疹病毒感染、人巨细胞病毒感染、PI3K-Akt信号通路为最显著的信号通路。结论黄芩通过31种活性成分作用于86个靶点来发挥降压的作用,反映了黄芩多成分、多靶点、多途径治疗高血压的特点。Objective To analyze the potential mechanism of Scutellariae Radix in the treatment of hypertension through network pharmacology.Methods The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)was used to screen the chemical components and targets of Scutellariae Radix;GeneCards,OMIM,TTD,and DrugBank databases were used to screen out the disease targets of hypertension;Cytoscape 3.7.2 software was used to construct the“active component-disease target”network of Scutellariae Radix in the treatment of hypertension.The protein-protein interaction(PPI)network was constructed with the help of String database;finally,the R language was used to perform GO annotation analysis and KEGG pathway analysis on the target.Results A total of 86 targets of interaction between Scutellariae Radix and hypertension were identified,among which,CCND1,CDKN1A,ESR1,and others were the core genes.GO functional enrichment mainly included cell response to chemical stress,oxidative stress and adrenergic receptor activity.Among the KEGG pathways,Kaposi sarcoma associated herpesvirus infection,human cytomegalovirus infection and PI3K-Akt signaling pathway were the most significant.Conclusion Thirty-one active components of Scutellariae Radix act on 86 targets to reduce blood pressure,which reflects the characteristics of Scutellariae Radix in the treatment of hypertension through multi-component,multi-target and multi-channel.

关 键 词：网络药理学 黄芩 高血压 潜在靶点 作用机制 

分 类 号：R285[医药卫生—中药学]