不同佐剂联合抗原表位融合蛋白对流感疫苗免疫原性的影响  

Effect of different adjuvants combined with epitope fusion protein on immunogenicity of influenza vaccine

作　　者：周云 方超 王丽 祁碧玉 金香玉 杨世龙 谭小东 ZHOU Yun;FANG Chao;WANG Li;QI Bi-yu;JIN Xiang-yu;YANG Shi-long;TAN Xiao-dong(Anhui Zhifei Longcom Biopharmaceutical Co.,Ltd.,Hefei 230088,Anhui Province,China)

机构地区：[1]安徽智飞龙科马生物制药有限公司,安徽合肥230088

出　　处：《中国生物制品学杂志》2021年第3期255-259,共5页Chinese Journal of Biologicals

基　　金：安徽省科技攻关计划项目“流感及结核疫苗研究与开发”(10140107008)。

摘　　要：目的观察不同佐剂与抗原表位融合蛋白配伍后对季节性流感疫苗在小鼠体内免疫原性的影响。方法流感病毒抗原表位融合蛋白(FP)分别辅以氢氧化铝、聚肌胞(PolyIC)+氢氧化铝以及阳离子佐剂免疫小鼠,再以四价流感病毒裂解疫苗(QIV)加强免疫,通过检测血凝抑制(hemagglutination inhibition,HI)效价和血凝素(hemagglutinin,HA)IgG抗体评价免疫原性。结果佐剂和融合蛋白本身并不能产生针对任何型别的HI抗体;QIV加强免疫后,阳离子佐剂配伍组显著提高了QIV同源株H1N1和H3N2型HI效价或阳转率,且提高了非同源株H3N2型HA IgG抗体水平。结论阳离子佐剂辅助流感病毒抗原表位融合蛋白免疫小鼠后,可显著增强季节性流感疫苗甲型毒株在小鼠体内的免疫原性。Objective To observe the effect of different adjuvants combined with epitope fusion protein(FP) on immunogenicity of seasonal influenza vaccines in mice. Methods Mice were primed with influenza virus epitope FP supplemented with aluminum hydroxide,PolyIC + aluminum hydroxide and cationic adjuvant respectively,then boosted with quadrivalent influenza virus split vaccine(QIV). The immunogenicity was evaluated by determination of hemagglutination inhibition(HI)titer and hemagglutinin(HA)IgG antibody. Results Adjuvant and FP produced no HI antibody of any types alone. However,after booster immunization with QIV,the HI titer or positive conversion rate against homologous strains of H1N1 and H3N2 types as well as the HA IgG antibody level against non-homologous strains of H3N2 type in the mice immunized with FP combined with cationic adjuvant increased significantly. Conclusion Influenza virus epitope FP combined with cationic adjuvant significantly enhanced the immunogenicity of seasonal influenza vaccine strain A in mice.

关 键 词：佐剂 流感疫苗 抗原表位 融合蛋白 免疫原性 

分 类 号：Q786[生物学—分子生物学] R392.7[医药卫生—免疫学]