高氏盆炎方五号方对慢性盆腔炎大鼠抗炎、抗粘连的作用及机制研究  

Experimental study on anti-inflammatory and anti-adhesive effects of No.5 Gao's Pelvic Inflammatory Prescription on rats with chronic pelvic inflammation

作　　者：王楚然 高慧 徐文君 郝艳红 陈十昔 WANG Churan;GAO Hui;XU Wenjun;HAO Yanhong;CHEN Shixi(2017 master's degree student,College of Traditional Chinese Medicine,North China University of Technology,Tangshan,Heibei 063000;不详)

机构地区：[1]华北理工大学中医学院,河北唐山063000 [2]河北省廊坊市妇幼保健中心中医科,河北廊坊065000 [3]承德医学院附属医院中医科,河北承德067000 [4]河北省秦皇岛市抚宁区人民医院内分泌肾病科,河北秦皇岛066300

出　　处：《河北中医》2021年第1期111-116,161,共7页Hebei Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局2014年全国名老中医药专家传承工作室建设项目(编号:2015048157);河北省中医药管理局2018年度中医药类科研计划课题(编号:2018164);河北省中医药管理局2017年度中医药类科研计划课题(编号:2017082)。

摘　　要：目的观察高氏盆炎方五号方对慢性盆腔炎(CPID)大鼠抗炎、抗粘连的作用及机制研究。方法将75只健康雌性SD大鼠随机抽取10只为空白组,其余65只通过苯酚胶浆及机械损伤法建立CPID模型,从中随机抽取5只大鼠确定造模成功。造模成功后,将其余60只大鼠随机分为6组,即模型组、少腹逐瘀颗粒组、人胎盘组织液组及高氏盆炎方五号方高、中、低剂量组,每组10只。空白组自由食水;模型组予0.9﹪氯化钠注射液1 mL/100 g灌胃;高氏盆炎方五号方高、中、低剂量组分别予高氏盆炎方五号方28、14、7 g/kg灌胃;少腹逐瘀颗粒组予少腹逐瘀颗粒1.5 g/kg灌胃;人胎盘组织液组予人胎盘组织液1 mL/kg后腿大腿肌肉处注射。均每日1次,连续20 d。给药结束后,取大鼠血清及子宫组织,光镜下观察子宫组织病理形态学变化,检测血清人成纤维细胞生长因子2(FGF-2)、白细胞介素2(IL-2)含量,测定子宫组织FGF-2蛋白和FGF-2基因表达。结果光镜下观察子宫组织病理形态学变化:模型组宫腔扩张处子宫壁变薄,增生明显处子宫壁增厚,腺体被破坏,有内膜上皮脱失现象,部分成纤维细胞增生,全层均见大量炎症细胞浸润。空白组子宫各层界限清楚,腺体规整,未见炎症细胞浸润。与模型组比较,高氏盆炎方五号方高、中剂量组宫腔扩张程度明显轻于模型组,上皮细胞形态趋于正常,内膜基本无充血水肿,成纤维细胞增生不明显,炎症细胞明显减少。高氏盆炎方五号方低剂量组宫腔扩张程度轻于模型组,肌层结构较紊乱,部分内膜充血、水肿,上皮细胞形态可见增生样变,较多炎症细胞浸润。少腹逐瘀颗粒组与高氏盆炎方五号方低剂量组相似。与空白组比较,模型组血清FGF-2水平及子宫组织中FGF-2蛋白表达的AOD和mRNA表达水平明显升高(P<0.05),血清中IL-2水平明显降低(P<0.05)。与模型组比较,高氏盆炎方五号方高、中、低�Objective To investigate the anti-inflammatory and anti-adhesive effect of No.5 Gao's Pelvic Inflammatory Prescription(#5-GPIP)on rats with chronic pelvic inflammatory disease(CPID).Methods Totally 75 healthy female SD rats were seleced,and 10 free rats as the blank group.The remaining 65 rats established CPID model through phenol mucilage and mechanical injury method,and 5 rats were randomly selected to confirm the model was successful.After successful modeling,the remaining 60 rats were randomly and equally divided into 6 groups;the model group(with gavaged 1 mL/100 g of 0.9%sodium chloride injection);The#5-GPIP groups of low-dose,medium-dose and high-dose(all gavaged 28,14,7 g/kg of#5-GPIP,respectively);Shaofu Zhuyu Granule group(with gavaged 1.5 g/kg of Shaofu Zhuyu);the human placenta tissue fluid group(with gavaged 1 mL/kg of human placental tissue fluid).It was administered once a day for 20 days.After the administration,the rat serum and uterine tissue were taken,and the pathological changes of the uterine tissue were observed under a light microscope,and the serum human fibroblast growth factor 2(FGF-2)and interleukin 2(IL-2)contents were detected.Measure FGF-2 protein and gene expression in uterine tissue.Results Pathomorphological change of uterine tissues was observed under the light microscope:In the model group,the uterine wall becomes thinner at the uterine cavity expansion,the uterine wall becomes thicker at the hyperplasia area,the glands are destroyed,there is the appearance of endometrial epithelial loss,some fibroblasts proliferate,and a large number of inflammatory cell infiltrations are seen in the whole layer.In the blank group,each layer of the uterus has clear boundaries,regular glands,and no inflammatory cell infiltration.Compared with the model group,the uterine cavity expansion in the#5-GPIP medium-dose and high-dose groups was significantly lighter,the morphology of epithelial cells tended to be normal,the intima was basically free of hyperemia and edema,the proliferation of fibroblas

关 键 词：盆腔炎性疾病 慢性病 中药疗法 实验 大鼠 

分 类 号：R711.33[医药卫生—妇产科学] R-05[医药卫生—临床医学]