TLR4/NF-κB信号通路在脊髓损伤中的作用  

Role of TLR4/NF-κB signaling pathway in pathogenesis of spinal cord injury

作　　者：王庆锋[1] 梁学刚 刘学起 WANG Qing-feng;LIANG Xue-gang;LIU Xue-qi(Department of Orthopedics,People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区人民医院骨科,宁夏银川750004

出　　处：《中国矫形外科杂志》2021年第6期531-535,共5页Orthopedic Journal of China

基　　金：宁夏回族自治区重点研发计划一般项目(编号:2018BE03046)。

摘　　要：[目的]探讨TLR4/NF-κB信号通路在急性脊髓损伤发病机制中的作用。[方法] SD大鼠随机分为假手术组、损伤组和单唾液酸神经节苷酯组(monosialoganglioside, GM1组)。其中,前一组仅行假手术,后两组采用改良Allen法建立大鼠脊髓损伤模型。术后,GM1组给予静脉GM1,假手术组、损伤组静脉给予等量生理盐水。术后15 d处死动物,行RT-qPCR和Western-blot检测TLR4/NF-κB和炎性因子的mRNA和蛋白的表达。[结果]与假手术组相比,损伤组的TLR4/NF-κB通路和相关炎性因子和mRNA和蛋白表达均显著增加(P<0.05)。与损伤组相比,GM1组的的TLR4/NF-κB通路和相关炎性因子和mRNA和蛋白表达显著下降(P<0.05)。[结论] TLR4/NF-κB信号通路可能成为治疗继发性脊髓损伤新的作用靶点,GM1主要通过抑制TLR4/NF-κB信号通路而起到治疗脊髓损伤的作用。[Objective] To explore the role of TLR4/NF-κB signaling pathway in the pathogenesis of acute spinal cord injury. [Methods] SD rats were randomly divided into sham operation group, injury group and monosialoganglioside group(GM1 group). Among them,the rats in the former one group had sham operation only, whereas the animals in latter two groups got spinal cord injury by the modified Allen method. After the operation, the rats in the GM1 group was given GM1 intravenously, whereas those in the sham operation group and the injury group were given the same amount of normal saline intravenously. The animals were sacrificed on 15 days after operation, and the spinal cord group was harvested for RT-qPCR and Western-blot assays of mRNA and protein expressions of TLR4/NF-κB signaling pathway and inflammatory factors. [Results] Compared with the sham operation group, the TLR4/NF-κB pathway and related inflammatory factors,mRNA and protein expression in the injury group were significantly increased(P<0.05). Compared with the injury group, the TLR4/NF-κB pathway and related inflammatory factors, mRNA and protein expression in the GM1 group were significantly decreased(P<0.05). [Conclusion] The TLR4/NF-κB signaling pathway may become a new target for the treatment of secondary SCI. GM1 mainly acts to treat SCI by inhibiting the TLR4/NF-κB signaling pathway.

关 键 词：脊髓损伤 TLR4/NF-κB信号通路 炎性因子 单唾液酸神经节苷脂 

分 类 号：R318[医药卫生—生物医学工程]