小鼠外周血淋巴细胞亚群在细粒棘球蚴包虫致敏反应中的作用  

Role of peripheral blood lymphocyte subsets in echinococcosis-induced sensitization in mice

作　　者：于晓东[1] 亚力·亚森 王佳玲 李孟 叶建荣[1] Yu Xiaodong;Yali·Yasen;Wang Jialing;Li Meng;Ye Jianrong(Department of Anesthesiology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Graduate School of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054 [2]新疆医科大学研究生学院,乌鲁木齐830054

出　　处：《中华微生物学和免疫学杂志》2021年第3期182-189,共8页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(81660522)。

摘　　要：目的建立细粒棘球蚴过敏反应BALB/c小鼠模型,研究和探讨淋巴细胞亚群在细粒棘球蚴致敏反应中的作用。方法从自然感染细粒棘球蚴的绵羊肝脏中提取原头蚴,培养40 d后,以50个微囊/鼠的剂量,通过腹腔注射接种BALB/c小鼠,对照组注射无菌生理盐水。感染6个月之后,每只小鼠按0.1 ml/10 g经腹腔注射羊源细粒棘球蚴粗制囊液致敏。对照组继续注射无菌生理盐水。激发过敏后1 h,根据评分将小鼠分为正常对照组、未致敏组、致敏组,每组各6只。取小鼠内眦静脉血,使用流式细胞仪检测B细胞、NK细胞、CD3^(+)/CD4^(+)/CD8^(+)T细胞,液相多重蛋白定量技术检测白细胞介素-4(interleukin-4,IL-4)、IL-6、IL-13的变化。结果与对照组比较,未致敏组的B细胞百分比显著升高(P<0.001),致敏组的B细胞百分比上升,但差异无统计学意义。与未致敏组比较,致敏组的B细胞百分比显著下降(P<0.01)。与对照组比较,未致敏组和致敏组的NK细胞百分比显著下降(P<0.001和P<0.01)。与未致敏组比较,致敏组的NK细胞百分比显著上升(P<0.05)。与对照组比较,未致敏组的CD3^(+)T细胞和CD4^(+)T细胞百分比显著降低(P<0.05),CD8^(+)T细胞百分比显著升高(P<0.01);致敏组的CD3^(+)T细胞和CD4^(+)T细胞百分比显著升高(P<0.05和P<0.001),CD8^(+)T细胞百分比无显著变化。与未致敏组比较,致敏组的CD3^(+)T细胞和CD4^(+)T细胞百分比显著升高(P<0.05),CD8^(+)T细胞百分比显著降低(P<0.01)。与对照组比较,未致敏组IL-4、IL-6、IL-13含量都显著升高(P<0.001);致敏组IL-4含量升高,但差异无统计学意义(P>0.05),IL-6含量显著升高(P<0.01),IL-13含量下降,但差异无统计学意义(P>0.05)。与未致敏组比较,致敏组IL-4、IL-6、IL-13含量都显著降低(P<0.001)。结论本研究成功建立了细粒棘球蚴过敏反应BALB/c小鼠模型,证明了Th2细胞引导的体液免疫应答在细粒棘球蚴囊液引起的过敏性休克�Objective To establish a BALB/c mouse model of Echinococcus granulosus allergy and investigate the role of lymphocyte subsets in Echinococcus granulosus-induced sensitization.Methods Echinococcus granulosus was isolated from the liver of sheep naturally infected with Echinococcus granulosus and cultured for 40 d.BALB/c mice were intraperitoneally injected with 50 microcapsules and sensitized by intraperitoneal injection of 0.1 ml/10 g of larval Echinococcus granulosus capsule six months after infection.According to the symptom scores 1 h after sensitization,these mice were divided into two groups:non-sensitized group(n=6)and sensitized group(n=6).The mice(n=6)in control group were injected with sterile saline.Blood sample was collected from the angular vein of each mouse.Flow cytometry was used to detect B cells,NK cells and CD3^(+)/CD4^(+)/CD8^(+)T cells.Cytometric bead array was used to measure IL-4,IL-6 and IL-13.Results The percentage of B cells was significantly higher in the non-sensitized group than in the control group(P<0.001),but no significant difference was observed between the sensitized group and the control group.Compared with the non-sensitized group,the percentage of B cells in the sensitized group decreased significantly(P<0.01).Compared with the control group,the percentages of NK cells in the non-sensitized group and the sensitized group decreased significantly(P<0.001 and P<0.01).Compared with the non-sensitized group,the percentage of NK cells in the sensitized group increased significantly(P<0.05).Compared with the control group,the percentage of CD3^(+)and CD4^(+)T cells in the non-sensitized group decreased significantly(P<0.05),while the percentage of CD8^(+)T cells increased significantly(P<0.01).Compared with the control group,the percentage of CD3^(+)and CD4^(+)T cells in the sensitized group increased significantly(P<0.05 and P<0.001),while no significant change in the percentage of CD8^(+)T cells was detected.Compared with the non-sensitized group,the percentage of CD3^(+)and CD4^(+

关 键 词：细粒棘球蚴 BALB/C小鼠 过敏反应 B细胞 NK细胞 CD3^(+)/CD4^(+)/CD8^(+)T细胞 

分 类 号：R532.32[医药卫生—内科学]