Foxp3^(+)Treg和IDO^(+)APC细胞在不同病变宫颈组织中的表达  

Expression of Foxp3^(+)Treg and IDO^(+)APC cells in different pathological cervical tissues

作　　者：马茜 田思娟[1] 赵娟 赵敏伊[1] 裴美丽 王丽[1] 杨筱凤[1] 杨婷[1] MA Qian;TIAN Sijuan;ZHAO Juan;ZHAO Minyi;PEI Meili;WANG Li;YANG Xiaofeng;YANG Ting(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China;Department of Gynecology,Yan'an Hospital Affiliated to Kunming Medical University,Yunnan Kunming 650051,China)

机构地区：[1]西安交通大学第一附属医院妇产科,陕西西安710061 [2]昆明医科大学附属延安医院妇科,云南昆明650051

出　　处：《现代肿瘤医学》2021年第9期1587-1592,共6页Journal of Modern Oncology

基　　金：陕西省重点研发计划项目(编号:2017SF-016)。

摘　　要：目的:检测免疫抑制细胞Foxp3^(+)Treg和免疫蛋白IDO在不同病变宫颈组织中的表达情况。方法:采用免疫细胞化学及蛋白印记(Western blot)检测Foxp3蛋白及IDO蛋白在宫颈癌细胞系HeLa、SiHa及C33A中的表达与分布;采用免疫组织化学法检测Foxp3和IDO在20例正常宫颈组织及45例不同程度宫颈病变组织中的表达情况。结果:Foxp3蛋白在HeLa、SiHa及C33A中无表达;IDO蛋白在HeLa、SiHa及C33A细胞的胞浆中均有表达。Foxp3蛋白在宫颈组织中表达于Treg,IDO蛋白在宫颈组织中表达于APC和/或异常细胞。宫颈病变组织中的Foxp3^(+)Treg(H=43.211,P=0.000)和IDO蛋白的表达均明显高于正常组织(χ^(2)=20.028,P=0.00;Z=226.600,P=0.00)。随着病理级别升高,Foxp3^(+)Treg的侵袭性(H=28.307,P=0.000)增加,Foxp3^(+)Treg与IDO^(+)APC的数目显著正相关(rs=0.550,P=0.003)统计学正相关(rs=0.550,P=0.000)。结论:在宫颈癌进展的不同病理阶段,局部微环境中Foxp3^(+)Treg数目随宫颈细胞恶性转化的进程而增多,IDO^(+)APC细胞在早期宫颈病变组织中表达多于晚期病变。Objective:To explore the expression and distribution pattern of Foxp3^(+)Treg and IDO protein in cervical carcinoma and its precancerous lesions.Methods:Western blot and immunocytochemistry(ICC)were conducted to investigate the expression of Foxp3 protein and IDO protein in three cervical cancer cell lines-HeLa,SiHa and C33A.Immunohistochemistry(IHC)was used to investigate their expression patterns in 45 cases of cervical lesions tissues of the cervix and 20 cases of normal cervix tissues.Results:Foxp3 protein was not expressed in HeLa,SiHA and C33A.IDO protein was expressed in the cytoplasm of HeLa,Siha and C33A cells,Foxp3 protein was expressed in cervical tissue and Treg protein,IDO protein was expressed in APC and abnormal cells in cervical tissue.Compared with normal cervix,the expression of Foxp3^(+)Treg(H=43.211,P=0.000)and IDO protein(χ^(2)=20.028,P=0.00;Z=226.600,P=0.00)were significantly increased in cervical lesions.Compared with low grade squamous cell carcinoma,the invasion of Foxp3^(+)Treg was increased in high grade squamous cell carcinoma(H=28.307,P=0.000).The results of Foxp3^(+)Treg investigation in different cervical lesions showed that Foxp3^(+)Treg expression was positively correlated with IDO protein(rs=0.550,P=0.000).Conclusion:In cervical immune microenvironment,Foxp3^(+)Treg and IDO protein which expressin the APC and abnormal differentiated cell may synergistically promote immunosuppression status,which plays an important role in the occurrence and development of tumor microenvironment milieu,especially in low grade cervical lesions.

关 键 词：宫颈癌 免疫微环境 TREG FOXP3 IDO 

分 类 号：R737.33[医药卫生—肿瘤]