二甲双胍联合抗阻运动对糖尿病大鼠下丘脑TGF-β1-Smad2/3的影响  

Effect of Metformin Combined with Resistance Exercise on TGF-β1-Smad2/3 in Hypothalamus of Diabetic Rats

作　　者：江秉泽 余思慧 张蕴南 纪梦迪 李佳欣 韦娟 JIANG Bingze;YU Sihui;ZHANG Yunnan;JI Mengdi;LI Jiaxin;WEI Juan(Sports Health Institute,Nanjing Sport Institute,Nanjing,Jiangsu Province,210014 China)

机构地区：[1]南京体育学院运动健康学院,江苏南京210014

出　　处：《当代体育科技》2021年第8期10-14,共5页Contemporary Sports Technology

基　　金：江苏省高等学校大学生创新创业训练计划项目(201810330041X)。

摘　　要：目的:探究二甲双胍联合抗阻运动干预对糖尿病大鼠的血糖的影响,以及对下丘脑TGF-β1、Smad2/3含量的影响。方法:采用高糖高脂饲料喂养结合腹腔注射链脲佐菌素(Streptozocin,STZ)注射液诱导糖尿病大鼠模型,二甲双胍和抗阻爬梯干预,Western-Blot法检测糖尿病大鼠下丘脑TGF-β1、Smad2/3蛋白表达量。结果:二甲双胍和运动均有一定降血糖效果,以药物治疗最为明显,联合干预的效果低于单独干预;二甲双胍和抗阻运动干预及其联合干预,Smad2/3、TGF-β1表达无明显的改变。结论:6周的糖尿病进程可能没有损伤大鼠下丘脑,抗阻运动和二甲双胍药物干预降低糖尿病大鼠血糖可能存在一定的抵消作用。Objective:To investigate the effect of metformin combined with resistance exercise intervention on blood glucose in diabetic rats,as well as the effect of hypothalamic TGF-β1 and Smad2/3 content.Methods:High-sugar and high-fat diet feeding combined with intraperitoneal injection of streptozocin(STZ)injection induced diabetic rat model,metformin and resistance ladder intervention,Western-Blot method to detect hypothalamic TGF-β1 in diabetic rats,Smad2/3 protein expression.Results:Metformin and exercise both have a certain hypoglycemic effect,and the most obvious effect is drug therapy,and the effect of combined intervention is lower than that of single intervention;metformin and resistance exercise intervention and their combined intervention,Smad2/3 and TGF-β1 expression have no signif icant changes.Conclusion:The 6-week course of diabetes may not damage the hypothalamus of rats.There may be a certain offsetting effect of resistance exercise and metformin drug intervention to reduce blood sugar in diabetic rats.

关 键 词：糖尿病 下丘脑 抗阻运动 TGF-Β1 SMAD2/3 

分 类 号：G804[文化科学—运动人体科学]