基于荧光光谱法的皮肤胆固醇快速无创检测技术  

Rapid Non-Invasive Technology for Skin Cholesterol Detection Based on Fluorescent Spectrometry

作　　者：吴鹏 倪敬书 洪海鸥 李晓静[3] 姚蓓 郑浩然[3] 花昌义 王霞 张元志 张洋 王贻坤 董美丽 Wu Peng;Ni Jingshu;Hong Haiou;Li Xiaojing;Yao Bei;Zheng Haoran;Hua Changyi;Wang Xia;Zhang Yuanzhi;Zhang Yang;Wang Yikun;Dong Meili(College of Physics and Electronic Information,Anhui Normal University,Wuhu,Anhui 241000,China;Anhui Provincial Engineering Laboratory for Medical Optical Diagmosis&Treatment Technology and Inst rument,Anhui Institute of Optics and Fine Mechanics,Hefei Institute of Physical Science,Chinese Academy of Sciences,Hefei,Anhui 230026,China;Health Management Centre,the First Affiliated Hospital of University of Science and Technology of China,Hefei,Anhui 230001,China;Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument,Wanjiang Center for Development of Emerging Industrial Technology,Tongling,Anhui 244000,China)

机构地区：[1]安徽师范大学物理与电子信息学院,安徽芜湖241000 [2]中国科学院合肥物质科学研究院安徽光学精密机械研究所,安徽省医用光学诊疗技术与装备工程实验室,安徽合肥230026 [3]中国科学技术大学附属第一医院健康管理中心,安徽合肥230001 [4]皖江新兴产业技术发展中心,安徽省生物医学光学仪器工程技术研究中心,安徽铜陵244000

出　　处：《中国激光》2021年第3期98-106,共9页Chinese Journal of Lasers

基　　金：中国科学院科技服务网络计划(KFJ-STS-QYZD-184,KFF-STS-ZDTP-063);安徽省科技重大专项(201903a07020027,17030901017,17030801007);中国科学院国际合作局对外合作重点项目(116134KYSB20170018);安徽省自然科学基金(1908085QH365);安徽省重点研发项目(202004a07020016,1804b06020350,1804h08020291)。

摘　　要：皮肤胆固醇含量可以作为评价动脉粥样硬化的重要指标之一,现有的皮肤胆固醇含量检测主要基于实验室活检进行,缺少快速无创的检测技术和装备。针对以皮肤胆固醇含量为评价指标的动脉粥样硬化的早期快速筛查需求,本文提出了基于荧光光谱法的皮肤胆固醇快速无创检测方法,研发了一种皮肤胆固醇无创检测系统。为了提高测量的准确性和稳定性,该系统对温度引起的检测试剂荧光效率的波动进行了修正。本文结合气相色谱法对测量结果的准确性进行了验证,并通过检测正常人群和动脉粥样硬化高风险人群的皮肤胆固醇含量,明确了该系统的临床应用价值。本文的研究结果表明,462~520 nm波段内的平均荧光强度与温度的相关系数为-0.995(p<0.0001),可据此建立温度校准曲线对由温度差异引起的荧光波动进行修正。校正后,系统测量的皮肤胆固醇含量与气相色谱测量值的相关性显著,相关系数为0.905(p<0.0001)。在动脉粥样硬化高风险人群的筛查实验中,动脉粥样硬化高风险人群和正常人群的皮肤胆固醇检测结果具有显著差异(P=0.0004)。与现有技术相比,基于荧光光谱法的皮肤胆固醇检测技术具有测量快速无创等优势,为大规模开展动脉粥样硬化的早期风险筛查提供了先进技术手段。Objective Skin cholesterol is an important biomarker for early atherosclerosis screening.Atherosclerosis is the leading cause of disability and death from cardiovascular disease.Effective control of pathogenic factors in the early pathological stage may delay or prevent the development of asymptomatic atherosclerosis into cardiovascular diseases.Thus,skin cholesterol detection becomes relevant in the prevention of cardiovascular diseases.Traditional skin cholesterol detection methods,such as skin biopsy or tape stripping,are invasive and usually time consuming.Alternatively,the recent three-drop method is being widely studied.In this method,three specific concentrations of reagents that bind to skin cholesterol are used on the skin surface of a subject,and atherosclerosis can be diagnosed by analyzing the reagent color changes.However,the three-drop method is sensitive to the application habits of the operator.Moreover,the detection reagents contain enzymes,polymers,and small molecule compounds,hindering quality control and increasing the sensitivity to environmental factors such as temperature and pH levels.We report a non-invasive skin cholesterol detection technique based on fluorescent spectrometry.By measuring the fluorescence spectrum of fluorescent-labeled skin,the cholesterol content can be calculated from the fluorescence spectra.This method corrects the influence of temperature on the test results and provides stability under various environmental conditions.Moreover,the skin cholesterol content can be obtained within 4 minutes.The proposed method provides a rapid non-invasive and stable method for skin cholesterol detection and corresponding applications including early atherosclerosis screening.Methods The proposed non-invasive skin cholesterol detection system is composed of a light source,fiber probe,spectrometer,photodiode,infrared temperature sensor,and computer.The fluorescence fluctuation of the detection reagent caused by temperature variation is corrected by establishing the relation between t

关 键 词：医用光学 皮肤胆固醇 荧光光谱法 无创检测 动脉粥样硬化 

分 类 号：TN29[电子电信—物理电子学]