基于R6G探针表面增强拉曼散射的pH传感  

pH Sensing Based on Surface-Enhanced Raman Scattering Using R6G Probe

作　　者：邓子昂 李姝洁 刘杰 欧全宏[1] 时有明[2] 刘刚[1] Deng Ziang;Li Shujie;Liu Jie;Ou Quanhong;Shi Youming;Liu Gang(School of Physics and Electronic In formation,Yunnan Normal University,Kunming,Yunnan 650500,China;School of Physics and Electronic Engineering,Qujing Normal University,Qujing,Yunnan 655011,China)

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明650500 [2]曲靖师范学院物理与电子工程学院,云南曲靖655011

出　　处：《中国激光》2021年第3期139-147,共9页Chinese Journal of Lasers

基　　金：国家自然科学基金项目(31760341);云南省高校科技创新团队支持计划项目。

摘　　要：用金纳米粒子(Au NPs)作为基底,基于罗丹明6G(R6G)探针分子拉曼特征峰随pH的变化,构建了一种具有表面增强拉曼散射(SERS)信号输出的pH传感器。由于(闭)开环形式下存在的(去)质子化R6G分子吸附于增强基底表面的倾斜度取向不同,R6G在不同pH下表现出了不同的SERS活性。根据寻找到的R6G在1363 cm-1和1314 cm-1位置处的SERS光谱峰面积比与pH的线性关系,设计出pH传感器。实验结果表明:R6G的SERS信号在室温下可以稳定2 h以上;当样品溶液pH在7和3之间转换时,传感器表现出了较好的恢复性。在pH检测过程中引入其他金属阳离子后,该探针表现出了对H+较好的选择性。通过检测实际样品的pH发现,该探针的分析性能良好,适于在酸性介质中检测pH。Objective In some research fields and practical applications,the accurate determination of pH is extremely important.For example,in biology,the change in intracellular pH plays an important role in cell growth,apoptosis,ion transport,homeostasis,and enzymolysis.There are three main methods for traditional detection of pH:pH indicator;pH test paper;glass pH electrode.The principles of the pH indicator and test paper are essentially the same,and the pH value is determined from color change after the reaction using the indicator and test paper.However,because of the color change,the pH value of the solution is determined according to a standard colorimetric card,which is easily affected by subjective consciousness.At the same time,the detection target range is small;for example,pH test paper cannot display the pH value of oil.Furthermore,the accuracy of the pH value is limited by the method used.The use of a glass pH electrode is the main method for accurately determining pH,but it is not suitable for long-term use owing to susceptibility to mechanical damage,electrical interference,long preparation time,complex calibration,and other reasons;moreover,the detection targets are limited.Therefore,it is necessary to find a simple,noncontact,and reliable method to detect pH.In this study,a pH optical probe with good sensitivity,selectivity,reversibility,and stability was constructed using SERS technology,Au NPs as reinforcement substrate,and R6 G as a probe molecule.Our basic strategy and research results are expected to be helpful for the combination of pH detection with other fields and the tracking of pH changes in single molecules and tissue cells.Methods In this study,Au NPs were used as the enhancement substrate and R6 G was used as the probe molecule to output SERS signals.The pH value of the sample solution was rapidly and conveniently measured.First,Au NPs were prepared via chemical reduction,and the silicon wafers were modified using the soaking method to obtain four types of dispersed distributions;the particl

关 键 词：光谱学 表面增强拉曼散射 PH传感器 罗丹明6G 金纳米粒子 

分 类 号：O433[机械工程—光学工程]