高效液相色谱手性柱拆分喹禾灵  

Enantiomeric Separation of Pesticide Quizalofop-ethyl by Chiral HPLC Stationary Column

作　　者：陆金富 沈静茹[1] 覃信铭 潘玉 李敏锐 Lu Jinfu;Shen Jingru;Qin Xinming;Pan Yu;Li Minrui(Key Laboratory of Analytical Chemistry of the State Ethnic Affair Commission,College of Chemistry and Materials Science,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学化学与材料科学学院分析化学国家民委重点实验室,湖北武汉430074

出　　处：《山东化工》2021年第7期92-93,共2页Shandong Chemical Industry

基　　金：中央高校基金资助项目(CZY20036)。

摘　　要：论文以β-环糊精有机骨架晶体材料构筑反相高效液相色谱(HPLC)手性固定相拆分手性农药喹禾灵。分别从流动相配比、流速和缓冲溶液pH值等几个方面探究拆分喹禾灵的最佳色谱条件。实验结果表明,以柠檬酸三乙胺(浓度20 mmol/L、pH值4.00)和甲醇为流动相,在柠檬酸三乙胺缓冲液和甲醇体积比为45%∶55%,流速0.5 mL/min,检测波长240 nm,柱温25℃条件下,喹禾灵对映体分离度R s为1.88,最大分离度可达2.65。本文建立了一种新的拆分喹禾灵两对映异构体的HPLC方法。In this paper,β-cyclodextrin organic framework materials was used to construct a reversed-phase high performance liquid chromatography(HPLC)chiral stationary phase and separate the chiral pesticide quizalofop-ethyl.The optimal chromatographic conditions for the separation of quizalofop-ethyl were explored from the aspects of mobile phase ratio,flow rate and buffer pH.The experimental results show that using triethylamine citrate(concentration 20 mmol/L,pH 4.00)and methanol as the mobile phase,the volume ratio of triethylamine citrate and methanol is 45%∶55%,and the flow rate is 0.5 mL/min.With determination of wavelength of 240 nm and column temperature of 25℃,the baseline resolution of quizalofop-ethyl enantiomers was achieved,with a resolution of 1.88,maximum resolution can reach 2.65.A new HPLC method for the resolution of quizalofop-ethyl enantiomers was established.

关 键 词：对映体拆分 高效液相色谱 β-环糊精有机骨架手性固定相 喹禾灵 

分 类 号：O657.7[理学—分析化学]