小白菜次生代谢物质对小菜蛾雌虫行为的调节作用  

Regulation of pakchoi’s secondary metabolites on the behavior of female Plutella xylostella(Lepidoptera: Plutellidae)

作　　者：王占娣 张啦 丁百莲 史云东 王雅妮 鲁广秋 贾琳 WANG Zhandi;ZHANG La;DING Bailian;SHI Yundong;WANG Yani;LU Guangqiu;JIA Lin(College of Chemistry Biology and Environment,Yuxi Normal University,Yuxi 653100,Yunnan Province,China;Liaoning Jinpin Biotechnology Co.,Ltd.,Shenyang 110179,Liaoning Province,China)

机构地区：[1]玉溪师范学院化学生物与环境学院,云南玉溪653100 [2]辽宁金品生物科技有限公司,沈阳110179

出　　处：《农药学学报》2021年第2期323-330,共8页Chinese Journal of Pesticide Science

基　　金：云南省地方高校基础研究联合专项(2018FH001-038);2019年云南省大学生创新创业训练计划项目"植物挥发物和信息素调节小菜蛾雌虫行为的研究"(2019B61)。

摘　　要：为明确小白菜次生代谢物质对小菜蛾行为的调节作用,采用热回流法提取小白菜中不同极性物质,利用Y-型嗅觉仪评价这些物质对小菜蛾雌虫行为的调节效应,采用气相色谱-质谱联用仪(GC-MS)鉴定提取物中各化合物的化学结构。结果显示:小白菜不同极性提取物对小菜蛾雌虫行为均有一定的调节作用,其中,低极性物质在高浓度时有引诱效果,高极性物质在中、低浓度时有引诱效果。对比小白菜无水乙醇提取物和蒸馏水提取物发现,低浓度时小白菜无水乙醇提取液对小菜蛾的活性低于蒸馏水提取液。GC-MS鉴定结果显示:小白菜蒸馏水提取液中含有异柠檬酸乙酯和9,12,15-十八碳三烯醛两种化合物,其中异柠檬酸乙酯含量较高;小白菜无水乙醇提取液中含有6种化合物,含量较高的为3,7,11,15-四甲基-2-十六烯-1-醇,其次为9,12,15-十八碳三烯酸,其中,9,12,15-十八碳三烯酸和9,12,15-十八碳三烯醛具有相同的母环结构。进一步研究表明,9,12,15-十八碳三烯酸和9,12,15-十八碳三烯醛可能是小白菜调节小菜蛾行为的潜在活性物质,可用于小菜蛾绿色防治产品的开发。To clarify the regulation of pakchoi’s secondary metabolites on the behavior of Plutella xylostella L., Various polar substances from pakchoi using the heat reflux method were extracted. The regulation of these substances on the behavior of female P. xylostella was evaluated with a Y-tube olfactometer, and the chemical structures in the extract were identified by GC-MS. The results showed that all the extracts with varying polarities of pakchoi regulated the behavior of female P. xylostella. The low-polarity substances attracted females at high concentrations, and the high-polarity substances lured females at medium and low concentrations. Comparing the anhydrous-ethanol extracts with the distilled-water extracts of pakchoi, the former had lower attractant activity to P. xylostella than the latter at low concentrations. The results of GC-MS identification showed that the distilled-water extracts of pakchoi contained two compounds, namely ethyl iso-allocholate and 9,12,15-octadecatrienal, among which the concentration of ethyl iso-allocholate was higher. The anhydrous-ethanol extracts of pakchoi contained six compounds, the substance with the highest concentration was 3,7,11,15-tetramethyl-2-hexadecen-1-ol, followed by 9,12,15-octadecene carbtrienoic acid, in which 9,12,15-octadecatrienic acid and 9,12,15-octadecatrienal had the same parent ring structure. The results showed that 9,12,15-octadecatrienic acid and 9,12,15-octadecatrienal were the substances with potential activities to regulate the behavior of P. xylostella on pakchoi, which could be used for the development of lead compounds for the green control of P. xylostella.

关 键 词：小白菜 次生代谢物质 9 12 15-十八碳三烯酸 9 12 15-十八碳三烯醛 小菜蛾 行为调控 绿色防控 

分 类 号：TQ453.4[化学工程—农药化工] S433.4[农业科学—农业昆虫与害虫防治]