己唑醇及其对映体对人体乳腺癌细胞的选择毒性及氧化损伤研究  

Study on the enantioselectivity cytotoxicity and oxidative damage of hexaconazole on MCF-7 cells

作　　者：兰阳 孙大利 庞俊晓 孙晓红[2] LAN Yang;SUN Dali;PANG Junxiao;SUN Xiaohong(College of Public Health/Key Laboratory of Environmental Pollution Monitoring and Disease of Ministry of Education,Guizhou Medical University,Guiyang 550025,China;College of Food Science,Guizhou Medical University,Guiyang 550025,China;Food and Pharmaceutical Engineering Institute,Guiyang Universtiy,Guiyang 550005,China)

机构地区：[1]贵州医科大学公共卫生学院/环境污染与疾病监控教育部重点实验室,贵阳550025 [2]贵州医科大学食品科学学院,贵阳550025 [3]贵阳学院食品与制药工程学院,贵阳550005

出　　处：《农药学学报》2021年第2期348-356,共9页Chinese Journal of Pesticide Science

基　　金：贵州省科学技术厅项目(黔科合平台人才[2017]5718);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]203)。

摘　　要：在对映体水平上研究己唑醇对人体乳腺癌细胞(MCF-7)的选择毒性及氧化损伤。以MCF-7细胞作为受试对象,采用Cell Counting Kit-8 (CCK-8)试剂盒测定经己唑醇对映体处理后的细胞毒性;采用氧化损伤相关试剂盒测定经己唑醇对映体处理后,细胞内乳酸脱氢酶(lactate dehydrogenase, LDH)释放量、活性氧(reactive oxygen species, ROS)产生量、超氧化物歧化酶(superoxide dismutase, SOD)和过氧化氢酶(catalase, CAT)的活性。结果表明:在10~160 mg/L范围内随着染毒质量浓度的增加,经过(-)-、(+)-和rac-己唑醇处理后的MCF-7细胞活性分别从85.24%、87.11%和103.87%降低至4.07%、5.11%和5.24%。其中,(-)-己唑醇对MCF-7细胞活性的抑制率最高,其次是(+)-和rac-己唑醇。氧化损伤检测结果显示,MCF-7细胞经20、40和80 mg/L的己唑醇对映体暴露后,细胞内LDH释放量和CAT酶活性随着己唑醇质量浓度的增加而逐渐增加,而ROS产生量和SOD酶活性则随着己唑醇质量浓度的增加呈现出先上升后下降的趋势。与细胞毒性结果相似,经3种形式的己唑醇处理后,(-)-己唑醇诱导的细胞氧化损伤程度最高,其次是(+)-和rac-己唑醇。本研究结果表明,己唑醇及其对映体在MCF-7细胞内的毒性和氧化损伤程度大小顺序为(-)-己唑醇>(+)-己唑醇> rac-己唑醇。研究结果可为探明己唑醇的细胞毒性机制和开展食品安全风险评估提供依据。In this work, the enantioselective toxicity and oxidative stress of hexaconazole on MCF-7 cells were investigated.. The activities of MCF-7 cells were tested after the exposure of hexaconazole enantiomers by CCK-8 kit. The changes of LDH release, ROS production, SOD and CAT enzyme activities were measured by oxidative damage kits. Results showed that cell activities decreased from85.24%, 87.11% and 103.87% to 4.07%, 5.11% and 5.24%, respectively, after the treatment of(-)-,(+)-and rac-hexaconazole in the range of 10-160 mg/L.(-)-Hexaconazole exhibited the highest inhibitory rate on MCF-7 cell activity, followed by(+)-and rac-hexaconazole. Oxidative damage results showed that after hexaconazole exposure at the concentrations of 20, 40 and 80 mg/L, the intracellular LDH release and CAT enzyme activity were gradually increased with the increase of the concentration, while ROS production and SOD activity were increased first and then decreased with the increase of the concentration. Similar to cytotoxicity results,(-)-hexaconazole induced the highest cell oxidative damage. In conclusion, the toxicity order to MCF-7 cells was(-)-hexaconazole >(+)-hexaconazole >rac-hexaconazole. The results of this study provided a basis for further exploring the cytotoxicity mechanism of hexaconazole and the food safety risk assessment.

关 键 词：己唑醇 对映体 人体乳腺癌细胞 细胞毒性 氧化损伤 

分 类 号：S482.2[农业科学—农药学] TQ450.26[农业科学—植物保护]