基于高通量测序技术的濒危植物黄瑞香Daphne giraldii Nitsche根部内生真菌多样性研究  

The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology

作　　者：贺元 慕祺瑞 白贞芳[1] 姜丹[1] 任广喜[1] 刘春生[1] HE Yuan;MU Qi-rui;BAI Zhen-fang;JIANG Dan;REN Guang-xi;LIU Chun-sheng(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《药学学报》2021年第3期872-878,共7页Acta Pharmaceutica Sinica

基　　金：2019年北京市双一流人才培养(北京市教学名师奖)项目(1000041510139);北京中医药大刘春生教学名师工作坊项目(1000062420397)。

摘　　要：通过研究濒危植物黄瑞香根部真菌种类的多样性,为濒危植物黄瑞香真菌库的建立及筛选促进黄瑞香生长和提高其活性成分祖师麻甲素含量的内生真菌提供科学依据。以甘肃、山西两省的9份野生黄瑞香植株为研究对象,利用Illumina高通量测序技术对其测序并进行生物信息学分析,共获得632766条有效序列,包括348个OTU,4门,20纲,48目,108科,154属,208种,丰度占比前3的真菌属之和能够占到总丰度的65%以上,最高可以达到98.4%,链格孢属(Altemaria)、茎点霉属(Phoma)为黄瑞香的核心菌属,链格孢属为优势属;黄瑞香根部内生真菌多样性丰富,不同产区内生真菌多样性依次为:甘谷县>五台县>宕昌县。In order to provide a scientific basis for the establishment of a Daphnes Cortex medicinal material fungus library and the screening of endophytic fungi that promote the growth of Daphnes Cortex and increase the content of daphnetin,we used Illumina high-throughput testing technology to analyze 9 Daphnes Cortex samples from Gansu and Shanxi provinces.A total of 632766 valid sequences were obtained,including 348 OTUs,4 phyla,20 classes,48 orders,108 families,154 genera,and 208 species.The sum of the first 3 fungal genera account for more than 65%of the total abundance,with the highest reaching 98.4%.Alternaria and Phoma are the main genuses of Daphne giraldii Nitsche,and Altemaria is the dominant genus.The endophytic fungi community of Daphnes Cortex is rich in diversity,and the order of fungal diversity in different producing areas is:Gangu County>Wutai County>Tanchang County.

关 键 词：高通量测序 黄瑞香 内生真菌 多样性 濒危植物 

分 类 号：R931[医药卫生—生药学]