表面活性剂对中性纤维素酶生物抛光的影响  

Effects of surfactants on the application of neutral cellulase biopolishing

作　　者：杜斌 刘营 王冠 刁洋洲 刘国红 陈云峰 苏丹 DU Bin;LIU Ying;WANG Guan;DIAO Yangzhou;LIU Guohong;CHEN Yunfeng;SU Dan(College of Chemical and Environmental Engineering,Wuhan Institute of Techenology,Wuhan 430073,China;SunHY Biotechnology Co.Ltd.,Wuhan 430074,China)

机构地区：[1]武汉工程大学化学与环境工程学院,湖北武汉430073 [2]武汉新华扬生物股份有限公司,湖北武汉430074

出　　处：《印染助剂》2021年第4期40-43,54,共5页Textile Auxiliaries

基　　金：武汉工程大学研究生教育创新基金项目给予的经济支持

摘　　要：主要研究几种不同类型表面活性剂对中性纤维素酶生物抛光效果的影响。以失重率为生物抛光效果指标进行单因素实验,最佳抛光条件:55℃、pH 6.5、中性纤维素酶0.1%(omf)、60 min、浴比1∶10。其中,BZK、M2001、NSF-7、APG、CAB-35与DIC促进生物抛光,强弱顺序为CAB-35、DIC、NSF-7、BZK、APG、M2001;AES、E-1310P抑制生物抛光,强弱顺序为AES、E-1310P;CAB-35和DIC(两性离子表面活性剂)增强酶液稳定性;APG和NSF-7(非离子表面活性剂)对酶液稳定性影响不大;M2001和BZK(阳离子表面活性剂)略降低酶液稳定性;E-1310P和AES(阴离子表面活性剂)大大降低酶液稳定性。The effects of several different types of surfactants on the application effect of neutral cellu⁃lase biopolishing were mainly studied.Using the rate of weight loss as the index of the biopolishing effect to perform the single factor analysis,and the optimal polishing application conditions were as follows:55℃,pH 6.5,neutral cellulase 0.1%(omf),60 min,bath ratio 1∶10.Among them,BZK,M2001,NSF-7,APG,CAB-35 and DIC could promote the biopolishing,the specific strength order was:CAB-35,DIC,NSF-7,BZK,APG,M2001.AES and E-1310P could inhibit biopolishing,and the specific strength order was:AES,E-1310P.CAB-35 and DIC(Zwitterionic surfactant)could enhance the stability of the enzyme solution,APG and NSF-7(non-ionic surfactant)had little effect on the stability of the enzyme solution,M2001 and BZK(cationic surfac⁃tant)slightly reduced the stability of the enzyme solution,while E-1310P and AES(anionic surfactant)greatly reduced the stability of the enzyme solution.

关 键 词：中性纤维素酶 表面活性剂 复配 生物抛光 

分 类 号：TS195.6[轻工技术与工程—纺织化学与染整工程]