气相色谱-三重四极杆质谱法测定水产暂养水中丁香酚和异丁香酚  

Determination of Eugenol and Isoeugenol in Temporary Aquaculture Water by Triple Quadrupole GC/MS

作　　者：刘朋宇 LIU Pengyu(Shenyang Institute for Food and Drug Control,Shenyang 110022,China)

机构地区：[1]沈阳市食品药品检验所,辽宁沈阳110022

出　　处：《现代食品》2021年第6期145-148,152,共5页Modern Food

摘　　要：本研究通过使用正己烷饱和的乙腈提取、乙腈饱和的正己烷净化,建立了测定水产暂养水中丁香酚和异丁香酚的气相色谱-三重四极杆质谱法。方法学验证表明,两种麻醉剂在20~500μg·L^(-1)浓度范围内线性良好,检出限为2μg·kg^(-1),定量限为5μg·kg^(-1)。通过进行3个浓度水平加标,每个浓度平行实验6次。结果表明,两种丁香酚类麻醉剂的回收率在85.6%~105.7%,相对标准偏差在1.2%~5.3%。该方法简便、灵敏度高、结果准确可靠,适用于水产暂养水中丁香酚和异丁香酚的测定。In this study,a triple quadrupole GC/MS method for the determination of eugenol and isoeugenol in temporary aquaculture water was established byextraction with n-hexane saturated acetonitrile and purification with n-hexane saturated acetonitrile.Methodological verification showed that the two anesthetics had good linearity in the concentration range of 20~500μg·L^(-1),with the limit of detection of 2μg·kg^(-1) and the limit of quantification of 5μg·kg^(-1).Three concentration levels were labeled,and each concentration was tested for 6 times in parallel.The results showed that the recoveries of the two eugenol anesthetics ranged from 85.6%~105.7%,and the relative standard deviations ranged from 1.2%~5.3%.The method is simple,sensitive,accurate and reliable.It is suitable for the determination of eugenol and isoeugenol in temporary aquaculture water.

关 键 词：暂养水 丁香酚 异丁香酚 气相色谱-三重四极杆质谱 

分 类 号：O657.63[理学—分析化学] TS254.7[理学—化学]