基于网络药理学的杞菊地黄丸治疗糖尿病合并高血压的机制研究  

Study on the mechanism of Qiju Dihuang Pills in the treatment of diabetes mellitus combined with hypertension based on network pharmacology

作　　者：张园 张世超 ZHANG Yuan;ZHANG Shichao(Department of Medical Equipment,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Liaoning Province,Shenyang110032,China)

机构地区：[1]辽宁中医药大学附属医院医疗设备处,辽宁沈阳110032

出　　处：《中国医药导报》2021年第11期4-9,F0003,共7页China Medical Herald

基　　金：国家“十二五”科技支撑计划项目(2014BAI10B00)。

摘　　要：目的运用网络药理学技术研究杞菊地黄丸治疗糖尿病合并高血压的作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)检索枸杞子、菊花、泽泻、熟地、山药、茯苓、山茱萸、丹皮的主要活性成分,并预测作用靶点。通过GeneCards、TTD以及DisGeNET数据库分别检索糖尿病、高血压的相关疾病靶点,通过PubChem及Swiss Target Prediction数据库筛选得到中药的作用靶点,病与药作用靶点取交集,筛选得到共同作用靶点。通过STRING平台构建蛋白质-蛋白质相互作用网络图,利用Cytoscape软件对该网络进行分析得到核心靶点。借助Metascape平台分析核心靶点的GO-BP生物功能和京都基因和基因组数据库(KEGG)通路富集。结果筛选得到杞菊地黄丸活性化合物100个、作用靶点890个,与糖尿病合并高血压共有靶点138个,其中核心靶点39个。GO-BP生物学功能富集功能包括血液循环、肽基丝氨酸磷酸化、跨膜受体蛋白酪氨酸激酶信号通路等。KEGG信号通路包括VEGF信号通路、ErbB信号通路、Toll样受体细胞信号通路等。结论本研究深度挖掘了杞菊地黄丸治疗糖尿病合并高血压多成分、多靶点和多通路的复杂机制,为今后进一步研究其治疗糖尿病合并高血压的基础实验提供了依据和参考。Objective To explore the mechanism of Qiju Dihuang Pills in the treatment of diabetes mellitus combined with hypertension by network pharmacology.Methods Through traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP),the main active components of Lycii Fructus,Chrysanthemi Flos,Alismatis Rhizoma,Rehmanniae Radix Praeparata,Dioscoreae Rhizoma,Poria,Corni Fructus,Moutan Cortex were searched,and the target prediction was carried out.The related targets of diabetes mellitus and hypertension were searched by GeneCards,TTD and DisGeNET databases.The targets of traditional Chinese medicine were screened by PubChem and Swiss target prediction database.The common targets were selected by intersection of disease and drug targets.The STRING platform was used to construct a protein-protein interaction network model,and the network was topologically analyzed using Cytoscape software to obtain the core target.GO-BP biofunctional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were performed on the core targets by Metascape software.Results After screening,Qiju Dihuang Pills contained 100 active compounds,890 targets,138 targets with diabetes mellitus and hypertension,and 39 core targets.The biological function enrichment function of GO-BP included blood circulation,peptidylserine phosphorylation,transmembrane receptor protein tyrosine kinase signaling pathway,etc.KEGG signaling pathway included VEGF signaling pathway,ErbB signaling pathway,Toll-like receptor cell signaling pathway,and so on.Conclusion This study deeply explores the complex mechanism of Qiju Dihuang Pills in the treatment of diabetes mellitus combined with hypertension with multiple components,multiple targets and multiple pathways,and provides basis and reference for further research on the basic experiments of Qiju Dihuang Pills in the treatment of diabetes mellitus combined with hypertension in the future.

关 键 词：杞菊地黄丸 网络药理学 糖尿病 高血压 靶点预测 

分 类 号：R544.1[医药卫生—心血管疾病]