IL-17信号通路在回归热神经性疏螺旋体病中的作用  

Roles of IL-17 signaling pathway in the development of relapsing fever neuroleptospirosis

作　　者：程美慧 陆巍 张静 丁开云 刘红旗[1] CHENG Meihui;LU Wei;ZHANG Jing;DING Kaiyun;LIU Hongqi(Infection and Immunity Laboratory,Institute of Medical Biology,Chinese Academy of Medical Science&Peking Union Medical College,Yunnan Province,Kunming650118,China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所感染和免疫实验室,云南昆明650118

出　　处：《中国医药导报》2021年第10期4-7,11,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81571549);中国医学科学院医学与健康科技创新工程(2016-I2M-3-026)。

摘　　要：目的研究白细胞介素(IL)-17信号通路在回归热神经性疏螺旋体病中的作用。方法将14只C57BL/6野生型小鼠分为两组,S组为6只,E组为8只;8只IL-17信号通路缺陷(IL-17-/-)小鼠为IL-17-/-组。三组小鼠通过腹腔注射感染回归热疏螺旋体DAH菌株后,观察小鼠体内菌血症、神经系统发病情况及特异性抗体的发生情况。比较E组和IL-17-/-组小鼠脊髓的病理变化。结果S组小鼠体内有效地复制扩增并引起菌血症,产生螺旋体特异性IgG和IgM,中枢神经系统疾病症状发病率达到100%。E组和IL-17-/-组小鼠体内的菌血症变化水平基本一致,且均能诱导E组和IL-17-/-组小鼠螺旋体特异性IgG和IgM产生。感染9~14 d IL-17-/-组感染回归热疏螺旋体后的中枢神经系统疾病评分低于E组,差异有统计学意义(P<0.05或P<0.01)。IL-17-/-组小鼠脊髓组织中小胶质细胞的数量少于E组小鼠。结论IL-17信号通路在回归热疏螺旋体感染诱导的中枢神经系统疾病中起重要作用。Objective To investigate the roles of interleukin(IL)-17 signaling pathway in the development of relapsing fever neuroborreliosis.Methods Fourteen C57BL/6 wild-type mice were divided into two groups,six mice in group S,eight mice in group E,and eight mice with IL-17-/-signaling pathway defect(IL-17-/-)were IL-17-/-group.Three groups of mice were infected by intraperitoneal injection of DAH strain of Borrelia recurrentis(B.recurrentis),and the occurrence of bacteremia in vivo,specific antibodies and the incidence of nervous system were observed.The pathological change of spinal cord was compared between group E and IL-17-/-group.Results The mice in group S were effectively replicated and amplified and bacteremia was induced,and spirochete specific IgG and IgM were produced,and the incidence of symptoms of central nervous system diseases reached 100%.The level of bacteremia in mice of group E and IL-17-/-group were basically the same,and the production of spirochete specific IgG and IgM could be induced in mice of group E and IL-17-/-group.The central nervous system disease scores of IL-17-/-group infected with B.recurrentis were lower than those of group E after 9-14 d of infection,and the differences were statistically significant(P<0.05 or P<0.01).The number of microglial cells in spinal cord tissue of mice in IL-17-/-group was less than that in group E.Conclusion IL-17 signaling pathway plays an important role in the development of central nervous system diseases induced by B.recurrentis infection.

关 键 词：IL-17信号通路 回归热 疏螺旋体 神经症状 

分 类 号：R514.1[医药卫生—内科学]