凡纳滨对虾(Litopenaeus vannamei)ATG3基因克隆及其在WSSV感染中的表达  

Cloning of ATG3 in Litopenaeus vannamei and research on its expression underWSSV infection

作　　者：尹文娟 刘芳 孟凡娟[1,2] 张广成 王丽燕 孙金生[1,2] YIN Wenjuan;LIU Fang;MENG Fanjuan;ZHANG Guangcheng;WANG Liyan;SUN Jinsheng(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2021年第2期51-55,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市自然科学基金资助项目(17JCYBJC29700);天津市水产生态及养殖重点实验室开放基金资助项目(53H15034);天津师范大学引进人才基金资助项目(5RL130).

摘　　要：为了揭示凡纳滨对虾自噬相关基因LvATG3在对虾先天免疫过程中的作用,利用PCR技术克隆出LvATG3,利用荧光定量PCR方法检测LvATG3在凡纳滨对虾不同组织中的表达,用白斑综合症病毒(WSSV)侵染凡纳滨对虾,检测WSSV侵染后不同时间LvATG3的基因表达情况.LvATG3 ORF区全长948 bp,编码315个氨基酸,蛋白相对分子质量为35.527×10^(3),对应等电点为4.58.LvATG3基因在凡纳滨对虾的眼柄、鳃、心脏、神经、血细胞等11种组织中均有表达,在中肠、后肠、鳃和血细胞中表达量较高.血细胞中,LvATG3的表达在WSSV病毒感染的不同时间呈现先下降再上升的趋势.凡纳滨对虾LvATG3参与的细胞自噬可能在WSSV病毒感染增殖过程中发挥了重要作用.To explain the function of autophagy related gene 3(LvATG3)of Litopenaeus vannamei in innate immune process,PCR technology was adopted to clone LvATG3,and fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression of LvATG3 in different tissues and in different time after infection of white spot syndrome virus(WSSV).The total length of LvATG3 ORF was 948 bp,encoding 315 amino acids.The molecular weight of predicted protein was 35.527×10^(3),and the corresponding isoelectric point was 4.58.LvATG3 gene was expressed in 11 tissues including eyestalk,gill,heart,nerve and haemocytes,etc.,and was highly expressed in midgut,hindgut,gill and haemocytes.In haemocytes,the expression of LvATG3 decreased first and then increased at different time of WSSV infection.LvATG3 of Litopenaeus vannamei and autophagy of this gene involved in may play an important role in the proliferation ofWSSV infection.

关 键 词：凡纳滨对虾 自噬 ATG3 白斑综合症病毒 

分 类 号：Q959.9[生物学—动物学]