地塞米松对人星形胶质细胞凋亡的影响  

Effects of dexamethasone on human astrocyte apoptosis

作　　者：董丽儒 杨仕坤 连俊波 霍双杰 罗丹 杨虎 宋旭东 丛斌 Dong Liru;Yang Shikun;Lian Junbo;Huo Shuangjie;Luo Dan;Yang Hu;Song Xudong;Cong Bin(Department of Pathology,Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,Hebei,China;Forensic Medicine of Hebei Medical University,Hebei Key Laboratory of Forensic Medicine,Shijiazhuang 050017,Hebei,China)

机构地区：[1]华北理工大学附属医院病理科,河北唐山063000 [2]河北医科大学法医学院,河北石家庄050017

出　　处：《中国法医学杂志》2021年第2期154-157,共4页Chinese Journal of Forensic Medicine

基　　金：河北省2018年度医学科学研究重点课题(20180767);国家自然科学基金重点项目(81430047)。

摘　　要：目的观察地塞米松(Dexamethasone,DEX)对人星形胶质细胞(Human astrocytes,HA)凋亡的影响。方法(1)培养人星形胶质细胞,免疫荧光法检测糖皮质激素受体(Glucocorticoid recepters,GR)的表达;(2)采用MTT实验检测不同浓度的DEX(10-9 M~10-5 M)对星形胶质细胞存活率的影响;(3)流式细胞术AnnexinⅤ-PI双标染色法检测细胞凋亡率。结果(1) HA细胞表达GR,阳性部位位于细胞浆;(2)与Con组相比,10-9 M~10-5M的DEX作用HA细胞48 h后,细胞存活率明显降低;(3)与Con组相比,10-6 M的DEX作用于HA细胞6h后,细胞凋亡率明显升高,DEX+RU486组与DEX组相比凋亡率明显下降。单独应用RU486组与CON组相比无明显差别。结论 DEX可通过与其受体结合独立诱导人星形胶质细胞凋亡。Objective To observe the apoptosis of human astrocytes(HA) by dexamethasone(DEX). Methods(1)Immumofluorescence method was used to examine the expression of GR in human astrocytes.(2) The effects of different concentrations of DEX(10-9 M~10-5 M) on astrocytes survival rate were determined by MTT.(3) Annexin V and PI staining was used to measure the apoptotic rates of astrocyte. Results(1) GR was localized dominantly in the cytoplasm in HA.(2)MTT results indicated that DEX(10-9 M~10-5 M) treatment gradually decreased cell viability compared with control group. 3. Compared with Control group, the apoptotic rate of HA was significantly increased after DEX treatment for 6 h, but the apoptotic rate of HA was decreased after co-treated with RU486. However, there is no significant difference between the Control group and RU486 group. Summary DEX can induce apoptosis of astrocytes independtely.

关 键 词：星形胶质细胞 凋亡 糖皮质激素 糖皮质激素受体 

分 类 号：DF795.1[医药卫生—法医学]