基于聚甲基丙烯酸保护的银纳米簇的双响应光谱法检测单质碘  

Optical Dual Response Detection of Iodine Based on Polymethacrylic Acid-Protected Silver Nanoclusters

作　　者：曹雪玲[1] 柏亚庚 朱琳[1] 李飞[1] 于晓洋[1] 于丽颖[1] CAO Xue-Ling;BAI Ya-Geng;ZHU Lin;LI Fei;YU Xiao-Yang;YU Li-Ying(College of Chemical&Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林132022

出　　处：《分析化学》2021年第5期759-767,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21605056);吉林省教育厅科学技术研究项目(No.JJKH20180553KJ)资助。

摘　　要：采用紫外光辐照法,一步制备了聚甲基丙烯酸保护的银纳米簇(AgNCs@PMAA),基于单质碘(I 2)与AgNCs@PMAA之间相互作用可引起荧光猝灭及特征吸收峰增强的原理,建立了荧光发射光谱-紫外可见吸收光谱法双响应检测单质碘的方法。形貌和光谱表征结果表明,制备的AgNCs@PMAA粒径均匀,单质碘对AgNCs@PMAA的融合作用可以引起其组成和形态变化。基于AgNCs@PMAA荧光发射峰及吸收峰强度变化与单质碘的浓度变化之间的线性关系,建立了两种检测单质碘的方法及可视化检测方法。以AgNCs@PMAA作为荧光探针检测I 2的线性范围为1.0~90.0μg/mL,检出限(S/N=3)为0.168μg/mL,方法具有良好的选择性。将此荧光探针用于碘酒中I 2的测定,加标回收率为100.5%~100.9%,相对标准偏差小于4.7%。此光学探针结合多种检测方法,具有良好的实际应用价值和应用前景。A polymethacrylic acid-protected silver nanocluster with fluorescence(AgNCs@PMAA)was prepared in one step using ultraviolet light irradiation.Based on the fact that the interaction between iodine and AgNCs@PMAA could cause fluorescence quenching and characteristic absorption,a dual response method for determination of iodine by fluorescence emission spectrometry and UV-vis absorption spectrometry was established.The prepared AgNCs@PMAA was characterized by TEM,XPS and FTIR,and the results showed that the AgNCs@PMAA had uniform small nanometer size and strong fluorescence luminescence,however,the fusion effect of iodine on AgNCs@PMAA could cause changes in its composition and morphology.Based on the linear relationship between the fluorescence emission peak and absorption peak intensity change of AgNCs@PMAA and the concentration of iodine elemental substance,two methods and visual detection method for detecting iodine were established and applied to actual sample determination.The fluorescence quenching efficiency of AgNCs@PMAA was linearly correlated with I 2 concentration within the range of 1.0-90.0μg/mL,and the detection limit was 0.168μg/mL(S/N=3).The fluorescent probe was applied to the determination of iodine in iodine tincture samples with recoveries of 100.5%-100.9%,and the relative standard deviations were less than 4.7%.The optical probe combined with multiple detection methods showed good practical significance and application prospects.

关 键 词：银纳米簇 光学探针 单质碘 紫外光辐照法 可视化检测 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]