雪松松针HPLC特征图谱及6个成分的含量测定  

HPLC characteristic chromatogram and assay of six components for pine needles of Cedrus deodara

作　　者：张国强[1] 李运 鲁艳梅 李婉玉 刘东彦[3] 石晓峰[1,2,3] 程显隆 ZHANG Guo-qiang;LI Yun;LU Yan-mei;LI Wan-yu;LIU Dong-yan;SHI Xiao-feng;CHENG Xian-long(Gansu University of TCM,Lanzhou 730030,China;Lanzhou Institute for Food and Drug Control,Lanzhou 730030,China;Gansu Academy of Medical Science,Lanzhou 730050,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]甘肃中医药大学,兰州730030 [2]兰州市食品药品检验所,兰州730030 [3]甘肃省医学科学研究院,兰州730050 [4]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2021年第3期525-532,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：2019年甘肃省省级引导科技创新发展专项资金(17);甘肃省科技支撑计划项目(1204FKCA152);兰州市人才创新创业项目(2014-RC-62)。

摘　　要：目的:利用高效液相色谱法建立雪松松针的特征图谱,同时测定其原儿茶酸、原儿茶醛、二氢杨梅素、咖啡酸、阿魏酸、芦丁6个成分的含量。方法:采用CAPCELL PAK-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇(A)-0.1%磷酸水(B)为流动相,梯度洗脱,流速为1 mL·min^(-1),检测波长300 nm,柱温30℃;应用中药色谱指纹图谱相似度评价软件(2012.130723版本)对14批雪松松针进行相似度分析,同时对其6个成分的含量进行测定。结果:14批雪松松针样品的色谱图与特征图谱的相似度介于0.932~0.992之间,标定共有峰22个,并对其6个共有峰进行归属。14批样品中原儿茶酸、原儿茶醛、二氢杨梅素、咖啡酸、阿魏酸、芦丁6个成分的含量范围分别为34.312~1216.750μg·g^(-1)、5.246~12.533μg·g^(-1)、43.912~132.047μg·g^(-1)、50.791~145.021μg·g^(-1)、13.543~70.379μg·g^(-1)、82.763~243.758μg·g^(-1),结论:所建立的HPLC特征图谱结合6个化学成分的含量测定,能较全面反映雪松松针内在化学成分质量,简便可行、快速准确,可为其质量控制提供参考。Objective:To establish an HPLC characteristic chromatogram of pine needles of cedrus deodara and to develop simultaneous determination method of protocatechuic acid,protocatechuic aldehyde,dihydromyricetin,caffeic acid,ferulic acid and rutin in the sample.Methods:HPLC analysis was performed on a CAPCELL PAK-C18 column(250 mm×4.6 mm,5μm).The mobile phase consisted of methanol(A)and 0.1% phosphoric acid aqueous solution(B)with gradient elution.The flow rate was 1 mL·min^(-1),the detection wavelength was 300 nm and the column temperature was 30℃.TCM chromatographic fingerprint similarity evaluation software(2012.130723 version)was used for similarity analysis in 14 batches of pine needles of Cedrus deodara,and the contents of six components in 14 batches of samples were determined at the same time.Results:The similarities between the characteristic chromatogram and chromatograms of 14 batches of pine needles in Cedrus deodara were between 0.932 and 0.992.There were altogether 22 common peaks,and six of them were identified.The contents of protocatechuic acid,protocatechuic aldehyde,dihydromyricetin,caffeic acid,ferulic acid and rutin in 14 batches of samples were in the range of 34.312^(-1)216.750μg·g^(-1),5.246^(-1)2.533μg·g^(-1),43.912^(-1)32.047μg·g^(-1),50.791^(-1)45.021μg·g^(-1),13.543-70.379μg·g^(-1) and 82.763-243.758μg·g^(-1),respectively.Conclusion:The combination of the HPLC characteristic chromatogram and content determination of six components can reflect inherent quality of pine needles of Cedrus deodara.The proposed method is simple,feasible,rapid and accurate,providing reference for quality evaluation of the drug.

关 键 词：雪松松针 特征图谱 原儿茶酸 原儿茶醛 二氢杨梅素 咖啡酸 阿魏酸 芦丁 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]