活血消异方治疗子宫内膜异位症网络药理学研究  

Study on Network Pharmacology of Huoxue Xiaoyi Formula in Treating Endometriosis

作　　者：刘哲[1,2] 赵瑞华 LIU Zhe;ZHAO Ruihua(Beijing University of Chinese Medicine,Beijing 100029,China;Guang’an Men Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]北京中医药大学,北京100029 [2]中国中医科学院广安门医院,北京100053

出　　处：《山东中医药大学学报》2021年第3期354-361,共8页Journal of Shandong University of Traditional Chinese Medicine

基　　金：首都卫生发展科研专项重点攻关项目(编号:首发2018-1-4151)。

摘　　要：采用网络药理学方法探析活血消异方治疗子宫内膜异位症(EMT)的相关靶点及作用机制。方法:通过中药系统药理学分析平台(TCMSP)及Swiss Target Prediction网络数据库推测并获取活血消异方组方的药物活性成分及靶点。利用基因卡片(GeneCard)数据库筛选获取EMT的疾病相关靶点,绘制药物成分-药物靶点网络、药物-疾病靶点的网络及核心蛋白质互作(PPI)网络,并对关键靶点进行京都基因与基因组百科全书(KEGG)和基因本体论(GO)基因富集分析。结果:从活血消异方中筛选出主要活性成分共99个,纳入靶点166个,其中疾病与药物的共同靶点66个;GO分析得到785项结果,主要涉及细胞的增殖、代谢、凋亡及激素受体活性等过程;KEGG分析得到124条通路,活血消异方治疗EMT主要涉及细胞增殖与凋亡、P53信号通路、磷脂酰肌醇-3-激酶/分子蛋白激酶B(PI3K/Akt)信号通路等。结论:活血消异方治疗EMT具有多成分、多靶点的特征,且涉及多条信号通路,此为进一步探讨该方治疗EMT机制提供了思路。Objective:To explore the relevant targets and action mechanism of Huoxue Xiaoyi Formula in the treatment of endometriosis(EMT)by network pharmacology.Methods:The active ingredients and targets of drugs in Huoxue Xiaoyi Formula were inferred and obtained through the Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP)and the Swiss Target Prediction network database.GeneCard database was used to screen and obtain disease-related targets of EMT After screening the targets,the drug component-drug target network,drug-disease target network and core protein protein interaction(PPI)network were drawn.Gene enrichment analysis was performed on key targets in Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO).Results:A total of 99 main active ingredients were screened from Huoxue Xiaoyi Formula,166 targets were included,there were 66 common targets for diseases and drugs;GO analysis yielded 785 results,mainly related to cell proliferation,metabolism,apoptosis and hormone receptor activity;KEGG analysis revealed 124 pathways.The treatment of EMT with Huoxue Xiaoyi Formula mainly involved cell proliferation and apoptosis,P53 signaling pathway,phosphatidylinositol-3-kinase-molecular protein kinase B(PI3K-Akt)signal pathways,etc.Conclusions:Huoxue Xiaoyi Formula has the characteristics of multiple components and multiple targets in the treatment of EMT,and involves multiple signal pathways.This provides ideas for further exploration of treatment mechanism of EMT by this Formula.

关 键 词：子宫内膜异位症 活血消异方 网络药理学 中药系统药理学分析平台 细胞增殖与凋亡 P53信号通路 磷脂酰肌醇-3-激酶/分子蛋白激酶B信号通路 

分 类 号：R271.113[医药卫生—中医妇科学] R243[医药卫生—中医学]