2020年新疆部分地区猪瘟病毒抗体检测及分析  

Detection and Analysis of Antibodies Against Classical Swine Fever Virus in Xinjiang in 2020

作　　者：孙琼飞 王紫阳 张小玉 党瑞莹 常军帅 屈勇刚[1,2] 李岩 SUN Qiongfei;WANG Ziyang;ZHANG Xiaoyu;DANG Ruiying;CHANG Junshuai;QU Yonggang;LI Yan(College of Animal Science and Technology,Shihezi University,Xinjiang Shihezi 832003,China;Key Laboratory of Control and Prevention of Animal Disease,Xinjiang Production&Construction Corps,Xinjiang Shihezi 832003,China;The General Station of Animal Husbandry and Veterinary Medicine of Xinjiang Production and Construction Corps,Urumqi 830063,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]动物疾病防控兵团重点实验室,新疆石河子832003 [3]新疆生产建设兵团畜牧兽医工作总站,新疆乌鲁木齐830063

出　　处：《中国猪业》2021年第2期56-58,62,共4页China Swine Industry

基　　金：八师石河子市促进科技成果转化引导计划(2019ZH05);石河子大学成果转化与技术推广计划(CGZH201908)。

摘　　要：为了解2020年新疆部分地区规模化猪场的猪瘟病毒(CSFV)抗体水平,本试验应用间接酶联免疫吸附试验(ELISA),对采自18个规模化猪场的1085份血清样品进行猪瘟病毒抗体检测。结果显示,CSFV抗体平均合格率为83.59%(907/1085),高于国家规定标准(70%),平均阻断率为(66.99±25.09)%。A、B、C、D、E地区间猪瘟病毒抗体水平差异显著(P<0.05)。各规模猪场猪群抗体合格率在70%~87%之间,并且差异显著。此次检测结果表明,6个不同地区平均抗体合格率中,A、B、C、F地区抗体水平整齐度良好,E地区抗体水平偏低,变异系数偏大,抗体整体水平较差。大型规模养猪场猪瘟抗体合格率普遍高于中、小型规模养猪场,说明大型规模养猪场在猪瘟的防控方面具有一定优势。该结果可为新疆地区规模化猪场的猪瘟免疫防控提供参考。In order to understand the antibody level against classical swine fever virus(CSFV)in some large-scale pig farms in Xinjiang in 2020,1085 serum samples from 18 large-scale pig farms were detected by indirect enzyme-linked immunosorbent assay(ELISA).The results showed that the average qualified rate of CSFV antibody was 83.59%(907/1085),which was higher than the national standard(70%),and the average blocking rate was(66.99±25.09)%.The antibody level against CSFV in A,B,C,D and E areas was significantly different(P<0.05).The qualified rate of antibody was between 70%and 87%,and the difference was significant.The test results showed that the antibody level homogeneity in A,B,C and F region was better,while the antibody level in E region was low,the coefficient of variation was large,and the antibody homogeneity was poor.The qualified rate of CSFV antibody in large-scale pig farms was generally higher than that in medium and small-scale pig farms,which indicated that large-scale pig farms had certain advantages in the prevention and control of CSFV.The results could provide a reference for the prevention and control of CSFV in scaled pig farms in Xinjiang.

关 键 词：猪瘟病毒 ELISA 抗体 检测 

分 类 号：S828[农业科学—畜牧学] S852.651[农业科学—畜牧兽医]