柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应  

Tendency response of Diaphorina citri Kuwayama to shoots and volatile components of twelve host plants

作　　者：汤夏安 李贤良 池章辰 韦舒蔚 邓业成[1] 邓志勇 骆海玉[1] 邓明学 TANG Xia-An;LI Xian-Liang;CHI Zhang-Chen;WEI Shu-Wei;DENG Ye-Cheng;DENG Zhi-Yong;LUO Hai-Yu;DENG Min-Xue(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education of China/College of Life Science,Guangxi Normal University,Guangxi Provice,Guilin 541004,China;Guangxi Academy of Specialty Crops,Guangxi Provice,Guilin 541004,China)

机构地区：[1]广西师范大学生命科学学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004 [2]广西特色作物研究院,广西桂林541004

出　　处：《环境昆虫学报》2021年第2期485-491,共7页Journal of Environmental Entomology

基　　金：广西科技重大专项(桂科AA18118046)。

摘　　要：为了发现对柑橘木虱Diaphorina citri Kuwayama具有引诱活性的寄主植物嫩梢挥发物,采用Y型嗅觉仪在室内测定柑橘木虱对12种寄主植物嫩梢及其挥发物成分的趋性反应,采用固相微萃取技术(SPME)和气-质联用分析技术(GC-MS)分析鉴定嫩梢挥发物的共同成分。结果表明,柑橘木虱对九里香Murraya exotica、沙田柚Citus maxima‘Shatian You’、红肉酸柚Cgrandis‘Hongrousuan You’、HB柚Citus grandis‘HB’、高斑柚Citus grandis‘gaoban You’和纽荷尔脐橙Citus sinensis‘Newhall’6种植物嫩梢具有显著的趋性反应,选择率分别为66.70%、66.04%、63.77%、63.86%、61.67%和60.35%。从寄主植物嫩梢中鉴定出8种挥发物共同成分,分别为α-石竹烯、(-)-β-石竹烯、萜品油烯、芳樟醇、(+)-α-蒎烯、3-蒈烯、D-柠檬烯和γ-松油烯。柑橘木虱对8种植物挥发物成分均有一定的趋性,其中对浓度为10μL/mL(-)-β-石竹烯和D-柠檬烯的选择率分别为73.20%和71.88%,对浓度为1μL/mL芳樟醇的选择率为79.19%。研究结果为进一步开发柑橘木虱引诱剂奠定了基础。In order to find the volatile components of shoots of the host plants with attractive activity to citrus psyllid Diaphorina citri Kuwayama,Y-type olfactory instrument was used to measure the tendency response of citrus psyllid to the shoots of twelve host plants and their volatile components,and solid phase microextraction(SPME)and gas-mass spectrometry(GC-MS)analysis were used to identify the common components of volatiles of the shoots.The results showed that the citrus psyllid had significant selective response to the shoots of Murraya exotica,Citrus maxima‘Shatian You’,Citrus grandis‘Hongrousuan You’,Citrus grandis‘HB’,Citrus grandis‘gaoban You’and Citrus sinensis‘Newhall’,and the selective rates were 66.70%,66.04%,63.77%,63.86%,61.67%and 60.35%,respectively.Eight common volatile constituents were identified from the shoots volatiles of host plants,namelyα-humulene,(-)-β-caryophyllene,terpinolene,linalool,(+)-α-pinene,3-carene,D-limonene andγ-terpinene.Citrus psyllid has selective response to the 8 plant volatile components.The selective rates to(-)-β-caryophyllene and D-limonene at 10μL/mL reach 73.2%and 71.88%respectively,and the selective rate to linalool at 1μL/mL reach 79.19%.The results laid the foundation for further development of attractants of citrus psyllid.

关 键 词：柑橘木虱 寄主植物 挥发物 趋性反应 

分 类 号：Q965[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]