丹皮酚对马铃薯块茎蛾雌虫产卵选择及幼虫的忌避作用研究  

Deterrent effect of paeonol on oviposition choice and larvae Phthorimaea operculella(Zeller)

作　　者：吴梅[1,2] 韩瑞 周典 刘燕 肖春[1] WU Mei;HAN Rui;ZHOU Dian;LIU Yan;XIAO Chun(Plant Protection College,Yunnan Agricultural University,Kunming 650201,China;College of Pharmaceutical Science,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南中医药大学中药学院,昆明650500

出　　处：《环境昆虫学报》2021年第2期500-506,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金(31560607)。

摘　　要：为探讨丹皮酚对马铃薯块茎蛾Phthorimaea operculella(Zeller)行为的影响,采用室内生物测定法,研究了丹皮酚对马铃薯块茎蛾雌虫产卵选择及初孵幼虫、老熟幼虫的忌避作用。结果表明,丹皮酚对马铃薯块茎蛾雌虫产卵、初孵幼虫、老熟幼虫均有显著忌避作用:选择性产卵实验中,5 mg/mL丹皮酚溶液的忌避指数为-0.63;非选择性产卵实验中,5 mg/mL和1 mg/mL丹皮酚溶液的产卵忌避率分别为-89.0%和-46.6%,且前者对马铃薯块茎蛾产卵有抑制作用;5 mg/mL及1 mg/mL丹皮酚溶液对马铃薯块茎蛾初孵幼虫均有极显著忌避作用,5 mg/mL丹皮酚溶液的忌避作用在0.5 h内可保持100%的忌避率,之后随时间延长而逐渐减弱;丹皮酚∶沙(1 mg∶2 g)对马铃薯块茎蛾老熟幼虫有显著忌避作用。本研究为丹皮酚作为一种有潜力的植物源忌避剂用于马铃薯仓贮期马铃薯块茎蛾虫害的防治提供理论依据。To investigate behavioral effect of paeonol on Phthorimaea operculella(Zeller),deterrent effect of paeonol on oviposition choice and larvae P.operculella were studied with bioassay methods in the laboratory.The results indicated that paeonol has significant deterrent effect on the oviposition,neogenetic larvae and mature larvae P.operculella.In the dual-choice oviposition test,oviposition deterrent index of 5 mg/mL paeonol solution was-0.63;in the no-choice oviposition test,deterrent rate were-89.0%and-46.6%of 5 mg/mL and 1 mg/mL paeonol solution respectively,and the former also inhibited oviposition of P.operculella;5 mg/mL and 1 mg/mL paeonol solution had significant deterrent effect on the neogenetic larvae,5 mg/mL paeonol solution could maintain 100%deterrent rate within 0.5 h,and then decreased with time;paeonol∶sand(1 mg∶2 g)had significant deterrent effect on the mature larvae.This experiment provides a theoretical basis for paeonol as a promising botanical deterrent in the P.operculella pest control during potato storage.

关 键 词：丹皮酚 牡丹皮 马铃薯块茎蛾 产卵选择 忌避 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]