海洋酸化和铜离子胁迫对海月水母碟状幼体的影响  

Effects of ocean acidification and copper ions on the ephyrae of moon jellyfish Aurelia coerulea

作　　者：王雷 彭赛君 董志军[1,3] WANG Lei;PENG Saijun;DONG Zhijun(Muping Coastal Environment Research Station,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China)

机构地区：[1]中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,烟台264003 [2]中国科学院大学,北京100049 [3]中国科学院海洋大科学中心,青岛266071

出　　处：《生态学报》2021年第4期1634-1644,共11页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(41876138);中国科学院科研仪器设备研制项目(YJKYYQ20180047);烟台市重点研发计划(2018ZHGY073)。

摘　　要：海洋酸化和重金属污染作为全球性海洋环境问题,均影响海洋生物的生存和生态系统的健康。海月水母是近岸海域常见的胶质性浮游动物,其生长发育和种群数量易受到海洋环境变化的影响。研究海月水母碟状幼体对海洋酸化(pH 8.1和pH 7.6)和Cu^(2+)(0、10μg/L和25μg/L)胁迫的生理响应,从氧化应激酶和酸碱平衡相关酶活性、呼吸、运动和生长等方面探究海洋酸化和铜污染对海月水母碟状幼体的综合影响。研究发现,经过16 d的暴露期后Cu^(2+)对海月水母碟状幼体产生较强的毒性效应,抑制Ca^(2+)-ATP酶和过氧化氢酶活性,造成超氧化物歧化酶活性和呼吸代谢速率异常升高、收缩频率降低、生长速度减慢。正常pH,25μg/L铜暴露处理的超氧化物歧化酶活性和呼吸率最高,收缩频率和伞部直径最小。随着铜暴露浓度的增加,海洋酸化可以缓解Cu^(2+)对海月水母呼吸率和生长的影响,但对Ca^(2+)-ATP酶和过氧化氢酶活性具有协同抑制作用。确定了海洋酸化和Cu^(2+)对海月水母的不同生理影响,这种差异影响可能会导致未来海洋生物多样性和生态系统的改变。Ocean acidification and heavy metal pollution are two global marine environmental problems,both of which have influences on the survival of marine organisms and the health of ecosystems.As a widely distributed glial zooplankton in the ocean,Aurelia coerulea plays an important role in biogeochemical cycle of important elements,such as carbon,nitrogen,and phosphorus.Many previous studies have reported the growth and population abundance of A.coerulea are susceptible to changes of the marine environment.In this study,the physiological response of A.coerulea ephyrae to ocean acidification(pH 8.1 and pH 7.6)and Cu^(2+)(0,10μg/L and 25μg/L)stress was analyzed by measuring the activities of physiological metabolic enzymes including catalase(CAT),superoxide dismutase(SOD),Ca^(2+)-ATPase,as well as respiration rates,pulsation rates and bell diameter of A.coerulea.The results showed that ocean acidification and Cu^(2+)stress had different effects on physiological indices of A.coerulea ephyrae.Cu^(2+)exposure can significantly inhibit the activities of CAT and Ca^(2+)-ATPase,as well as the pulsation rates and growth rate of A.coerulea ephyrae.Besides,Cu^(2+)exposure can also lead to a significant increase in SOD activity and respiratory rate.While copper pollution might affect the swimming behavior of A.coerulea,leading to the significant decline in their predation ability and ontogeny.Furthermore,a negative correlation was observed between the bell diameter and Cu^(2+)concentration.Seawater acidification had an inhibitory effect on activity of CAT,SOD,and Ca^(2+)-ATPase,as well as growth of A.coerulea ephyrae,and promoted its respiration.However,when A.coerulea ephyrae were exposed to co-exposure of seawater acidification and Cu^(2+),there were significant antagonistic effect between seawater acidification and Cu^(2+)on the growth and respiration of A.coerulea ephyrae.Our study indicates that the adaptability of A.coerulea ephyrae to copper pollution is enhanced with the background of ocean acidification.The different ph

关 键 词：海月水母 碟状幼体 海洋酸化 铜离子 生理响应 

分 类 号：S917.4[农业科学—水产科学]