Current Developments in the Stable Production of Human Induced Pluripotent Stem Cells  

作　　者：Ikki Horiguchi Masahiro Kino-oka 

机构地区：[1]Department of Biotechnology,Graduate School of Engineering,Osaka University,Osaka 565-0871,Japan

出　　处：《Engineering》2021年第2期144-152,共9页工程（英文）

基　　金：This research was supported by Japan Aency for Medical Research and Development(AMED,JP20be0604001).

摘　　要：Induced pluripotent stem cells(iPSCs)are considered to be ideal and promising cell sources for various applications such as regenerative medicine and drug screening.However,effective mass production systems for the stable supply of desired numbers of iPSCs are yet to be developed.This review introduces the various approaches that are currently available for stable iPSC production.We start by discussing the limiting factors to be controlled during iPSC culture,such as nutrient supply,waste removal,and oxygen availability.We then introduce recent investigations on iPSC culture systems based on adhesion,suspension,and scaffolds.We also discuss the downstream processes that follow the culture process,such as filling and freezing processes,which limit the production scale due to decreased cell viability during suspension in cryopreservation medium.Finally,we summarize the possibility of the stable mass production of iPSCs and highlight the limitations that remain to be overcome.We suggest that multidisciplinary investigations are essential to understand the different factors that influence cell growth and quality in order to obtain an optimal and stable iPSC mass production system.

关 键 词：Induced pluripotent stem cell Cell manufacturing EXPANSION FILLING FREEZING 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]