盐穗木叶片及根际土壤微生物群落高通量分析  

Analysis of Microbial Communities in Leaves and Rhizosphere Soil of Halostachys capsica by High-throughput Sequencing

作　　者：迪拉热·海米提 樊永红[1] 王伟楠 喻文丽 艾海白尔·卡斯木 Dilare Haimiti;FAN Yonghong;WANG Weinan;YU Wenli;Aihaibaier Kasimu(College of Life Science and Technology, Xinjiang University, Urumqi 830046, China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《新疆农业科学》2021年第4期731-740,共10页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金“新疆盐穗木根际ACC脱氨酶耐盐碱菌株的筛选及促生作用的研究”(31560032)。

摘　　要：【目的】研究新疆盐碱环境中,极端耐盐植物盐穗木的根际土壤及叶片内生微生物群落组成和结构,为利用根际促生细菌制作生物菌肥提供科学依据。【方法】应用16S rDNA和ITS高通量测序技术,测定分析盐穗木根际土壤与叶片内生微生物群落结构及多样性。【结果】在门水平上根际土壤细菌群落中的优势菌门为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、芽单胞菌门(Gemmatimonadetes)等。根际盐穗木真菌群落中的优势菌门为子囊菌门(Ascomycota)。在门水平下,盐穗木叶片内生细菌群落中的优势菌门为蓝细菌门(Cyanobacteria)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria);内生真菌群落中的优势菌门为子囊菌门(Ascomycota)。Alpha多样性指数分析得出,在3个土壤样品中,YS1的Shannon,Simpson指数最高,即微生物群落多样性较其余2个样品高。【结论】在3个叶片样品中,YP3的Shannon,Simpson等指数最高,即微生物群落多样性较其余2个样品高。盐穗木根际土壤微生物群落多样性高于叶片内生微生物群多样性。【Objective】To explore the rhizosphere soil and endophytic microbial community composition and structure of the extremely salt-tolerant plant Halostachys capsica in the saline-alkali environment of Xinjiang.【Methods】16 S rDNA and ITS high throughput sequencing technology were used in this project to determine and analyze the structure and diversity of endophytic microbial community in rhizosphere soil and leaves of salt panicle.【Results】The results showed that the dominant bacteria in the rhizosphere soil bacterial community at the level of the gate were Actinobacteria,Proteobacteria,and Gemmatimonadetes.The dominant bacterium in the rhizosphere was Ascomycota.At the level of the gate,the dominant bacteria in the endophytic bacterial community of the leaves of the leaves were Cyanobacteria,Actinobacteria,Proteobacteria,and a dominant bacterium in endophytic fungi was Ascomycota.According to the Alpha diversity index analysis,the Shannon and Simpson indices of YS1 were the highest in the three soil samples.Namely,the microbial community diversity was higher than the other two samples.【Conclusion】Among the three leaf samples,the Shannon,Simpson and other indexes of YP3 were the highest,that was,the microbial community diversity was higher than the other two samples.The microbial community diversity in the rhizosphere soil of Halostachys capsica was higher than that in the endogenous microbial community.To sum up,the analysis of microbial community composition in rhizosphere soil and leaves of Halostachys capsica provides scientific basis for further study of the correlation between microbial community and its resistance to stress in arid and saline-alkali habitats by PGPR.

关 键 词：盐穗木 根际微生物 内生菌 微生物群落多样性 高通量测序 

分 类 号：S188[农业科学—农业基础科学]