抗疏健骨颗粒对去卵巢骨质疏松大鼠血清骨代谢及骨组织自噬水平的影响  

Effect of Kangshujiangu Granules on the serum metabolism and the bone autophagy in ovariectomized rats with osteoporosis

作　　者：王玥 刘启玲 徐守竹 田敏敏 张蓓 张荣强 孙娜 史传道 WANG Yue;LIU Qiling;XU Shouzhu;TIAN Minmin;ZHANG Bei;ZHANG Rongqiang;SUN Na;SHI Chuandao(Department of Public Health,Shanxi university of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学公共卫生学院,陕西咸阳712046

出　　处：《中国骨质疏松杂志》2021年第4期487-491,507,共6页Chinese Journal of Osteoporosis

基　　金：陕西省科技厅社会发展领域项目(2020SF-206);国家自然科学基金青年项目(81901269);陕西中医药大学慢性非传染性疾病创新团队(132041933);陕西省教育厅自然面上项目(19JK0228)。

摘　　要：目的研究抗疏健骨颗粒对去卵巢骨质疏松(osteoporosis,OP)大鼠骨稳态及自噬相关因子的影响,探讨抗疏健骨颗粒干预OP的作用机制。方法将60只SD大鼠随机分为5组:假手术组、模型组、抗疏健骨颗粒低、中、高剂量组。除假手术组外,其余4组建立去卵巢大鼠OP模型。正常喂养12周检测模型建成后,给予抗疏健骨颗粒干预12周,采用ELLSA法测定血清ALP、BGP和TRACP水平和股骨GPR48、ATF4含量;蛋白印迹法(Western blotting)检测股骨中AMPK、LC3、Beclin1和P62蛋白表达。结果抗疏健骨颗粒可显著降低OP大鼠血清ALP、BGP和TRACP水平,升高股骨GPR48、ATF4含量,上调AMPK磷酸化水平及LC3、Beclin1蛋白表达,降低P62水平,差异有统计学意义(P<0.05)。抗疏健骨颗粒各剂量组中,中剂量组效果显著,与其他两组相比,差异有统计学意义(P<0.05)。结论抗疏健骨颗粒可调节血清、骨组织中骨代谢指标,减轻去卵巢大鼠OP的发生,其机制可能与通过骨组织中AMPK调节自噬通路有关,通过恢复自噬水平,促进骨生成,进而维持骨平衡。Objective The present investigation evaluated the effect of Kangshujiangu Granules on bone homeostasis and autophagy-related factors in ovariectomized osteoporosis(OP) rats and also postulated the possible mechanism of action.Methods Sixty SD rats were randomly divided into 5 groups: sham group,model group,and the low,medium and high dose groups of Kangshujiangu granules.Except for the sham group,the other 4 groups established OP models by ovariectomized.After 12 weeks,check the model construction,confirmed the model is built,the Kangshujiangu granules are given for 12 weeks.The level of ALP,BGP and TRACP in serum and GPR48,ATF4 levels in femoral were assessed by the ELISA.Western blotting was used to detect the expression of AMPK,LC3,Beclin1 and P62 proteins in the femur.Results Kangshujiangu Granules could significantly reduce serum ALP,BGP and TRACP levels in OP rats,increase the levels of GPR48,ATF4 levels in femoral,up-regulate AMPK phosphorylation levels and LC3 and Beclin1 protein expressions,and reduce P62 levels.The difference is statistically significant(P<0.05).The middle-dose group had a significant effect compared with the other two groups,the difference was statistically significant(P<0.05).Conclusion Kangshujiangu Granules can regulate bone metabolism indexes of serum and bone tissue,alleviate the symptoms of OP in ovariectomized rats.The mechanism may be related to the regulation of AMPK autophagy pathway,which can improve bone homeostasis by promoting bone formation.

关 键 词：中医中药 抗疏健骨颗粒 骨质疏松 自噬 AMPK 

分 类 号：R274[医药卫生—中医骨伤科学]