通过Micro-CT评价瘦素受体缺乏的2型糖尿病小鼠四肢骨的骨代谢变化  

The changes of bone metabolism in the extremities of type 2 diabetic mice with leptin receptor deficiency were evaluated by micro-CT

作　　者：何琪 杨均政 张罡瑜 潘兆丰 苏丽君 王海彬[2] 陈鹏[2] HE Qi;YANG Junzheng;ZHANG Gangyu;PAN Zhaofeng;SU Lijun;WANG Haibin;CHEN Peng(Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Orthopedics Department,First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国骨质疏松杂志》2021年第4期492-497,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81774339,82074462);广东省科技计划项目(2017A020213030);广东省中医药管理局科研项目(20191101)。

摘　　要：目的探讨瘦素受体(leptin receptor,Lepr)缺乏的2型糖尿病小鼠的骨骼表型,为2型糖尿病(T2DM)合并骨质疏松(OP)的防治提供一个新的靶点。方法获取20只14周龄雄性db/db小鼠(瘦素受体缺乏小鼠)和野生型小鼠(C57BL小鼠)的胫骨(各10只),通过Micro-CT检测比较两者骨小梁相对体积(BV/TV)、骨表面积组织体积比值(BS/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)、骨小梁分离度(Tb.Sp)、骨结构模型指数(SMI)、骨皮质厚度(Ct.Th)、骨皮质面积(Ct.Ar)等骨微结构参数的差异。结果与野生型小鼠相比,14周龄的db/db小鼠的胫骨骨小梁相对体积(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)明显减小,小梁骨间距(Tb.Sp)相应增加,皮质骨厚度(Ct.Th)、横截面积(Ct.Ar)减小,两者比较差异均有显著统计学意义(P<0.05);其结构模式指数(structure model index,SMI)较野生型明显减小,两者比较差异均有统计学意义(P<0.05)。结论 2型糖尿病可能是通过瘦素受体参与的信号通路影响了骨量变化,为利用该模型进行DOP病因及治疗方法研究提供了新的方向。且在缺乏瘦素信号传导的情况下,骨质量和强度的降低验证了瘦素在体内起着合成代谢骨因子的作用。Objective To investigate the skeletal phenotype of type 2 diabetic mice with Leptin receptor(Lepr) deficiency and to provide a new target for the prevention and treatment of type 2 diabetes mellitus(T2 DM) combined with osteoporosis(OP).Methods Get 20 males only 14 weeks of db/db mice(lack of leptin receptor) in mice and wild-type mice(C57 BL mice) shin(10),through the Micro-CT detection to compare both trabecular bone volume(BV/TV) relatively,the surface area of bone tissue volume ratio(BS/TV),bone trabecular thickness(Tb.Th),bone trabecular number(Tb.N),separating degree(Tb.Sp),trabecular bone bone structure model index(SMI),bone cortical thickness(Ct.Th),bone cortex area(Ct.Ar) bone microstructure parameters,such as difference.Results Compared with wild type mice,14 weeks of db/db mice tibial trabecular bone volume(BV/TV) and bone trabecular thickness(Tb.Th),bone trabecular number(Tb.N) significantly reduced,trabecular bone spacing(Tb.Sp) increased,the corresponding cortical thickness(Ct.Th),cross-sectional area(Ct.Ar) decreased,and compared the differences between all had significant statistical significance(P<0.05);The structure model index(SMI) was significantly lower than that of the wild type,and the difference between the two was statistically significant(P<0.05).Conclusion Type 2 diabetes may affect the change of bone mass through the signal pathway involved in leptin receptor,which provides a new direction for the study of DOP etiology and treatment method by using this model.In addition,in the absence of leptin signal transduction,bone mass and strength decrease,which proves that leptin plays a role of anabolic bone factor in the body.

关 键 词：瘦素受体 MICRO-CT 胫骨 2型糖尿病 骨质疏松 骨代谢 

分 类 号：R681[医药卫生—骨科学]