多囊卵巢综合征患者血清AQP8、AQP9水平与IVF-ET助孕结局  

The relationships between the serum AQP8 and AQP9 levels and the outcomes of women with PCOS after IVF-ET

作　　者：王婧彦[1] 管一春[1] 于晓娜[1] 张文[1] WANG Jingyan;GUAN Yichun;YU Xiaona;ZHANG Wen(The Third Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan Province,453002)

机构地区：[1]郑州大学第三附属医院,郑州453002

出　　处：《中国计划生育学杂志》2021年第4期789-793,共5页Chinese Journal of Family Planning

摘　　要：目的:探讨多囊卵巢综合征(PCOS)患者血清水通道蛋白(AQP)8、AQP 9水平与体外受精-胚胎移植(IVF-ET)助孕结局的关系。方法:回顾性收集本院2017年12月—2019年10月PCOS不孕行IVF-ET患者156例临床资料,根据助孕结局分妊娠组(n=70)和未妊娠组(n=86)。收集年龄、体质指数、不孕年限、基础促卵泡刺激素(FSH)、促黄体生成素(LH),雌二醇(E2)、孕酮(P)、睾酮(T)、泌乳素(PRL)、促性腺激素(Gn)用量、使用时间;同时记录获卵数、成熟卵率、受精率、优质胚胎率;实时荧光定量PCR检测血清AQP8、AQP9水平;Spearman法分析IVF-ET助孕妊娠者血清AQP8、AQP9水平与T、成熟卵率、受精率、优质胚胎率关系,logistic分析影响PCOS患者IVF-ET助孕妊娠的因素。结果:与未妊娠组相比,妊娠组血清中T、AQP8水平降低,血清AQP9、成熟卵率、受精率、优质胚胎率升高(均P<0.05)。Spearman法分析显示,IVF-ET助孕妊娠者血清AQP8水平与T呈正相关,与成熟卵率、优质胚胎率呈负相关(P<0.05);AQP9与T呈负相关,与受精率、优质胚胎率呈正相关(P<0.05)。AQP8、T是IVF-ET助孕结局妊娠的独立危险因素,AQP9、优质胚胎率是保护因素(均P<0.05)。结论:PCOS患者IVF-ET助孕妊娠者血清AQP8低表达、AQP9高表达,与IVF-ET结局指标关系密切,提示临床有一定意义。Objective: To investigate the relationship between the levels of serum aquaporin 8(AQP 8) and AQP 9 of women with polycystic ovary syndrome(PCOS) and their outcomes after in vitro fertilization and embryo transfer(IVF-ET). Methods: The clinical data of 156 infertile women with PCOS after IVF-ET from December 2017 to October 2019 were collected retrospectively. These women were divided into group A(70 women with pregnancy) and group B(86 women without pregnancy) according to the outcomes after IVF-ET. The general data were collected, which included the age, the body mass index, the infertility duration, the values of basic follicle stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E2), progesterone(P), testosterone(T), and prolactin(PRL), dosage and time of gonadotropin(Gn) used. At the same time, the number of obtained eggs, the rate of mature eggs, the rate of fertilization, and the rate of high-quality embryos were recorded. The levels of serum AQP8 and AQP9 were detected by real-time fluorescence quantitative PCR(qRT-PCR). Spearman method was used to analyze the relationship between the serum AQP8 and AQP9 levels of the women with pregnancy after IVF-ET and their T level, and the rates of mature egg, fertilization, and high-quality embryo. Logistic analysis was used to analyze the factors influencing pregnancy of the women with PCOS after IVF-ET. Results: Compared with those of the women in group B, the serum T and AQP8 levels of the women in group A were significant lower, while the serum AQP9 level, and the rates of mature egg, fertilization rate, and high-quality embryo of the women in group A were significant higher(all P<0.05). Spearman analysis showed that the serum AQP8 level of the women with pregnancy after IVF-ET was positively correlated with their T level,and was negatively correlated with the rates of mature eggs and high-quality embryos(P<0.05).The AQP9 level of the women with pregnancy after IVF-ET was negatively correlated with their T level,and was positively correlated with their r

关 键 词：多囊卵巢综合征 体外受精-胚胎移植 水通道蛋白 助孕结局 

分 类 号：R711.75[医药卫生—妇产科学]