盐穗木根际产ACC脱氨酶耐盐菌株的筛选及鉴定  

Screening and identification of salt-tolerant and ACC deaminase-producing strains in Halostachys caspica rhizosphere

作　　者：王伟楠 兰智勇 喻文丽 孙庆培 李佩琪 樊永红[1] WANG Wei-nan;LAN Zhi-yong;YU Wen-li;SUN Qing-pei;LI Pei-qi;FAN Yong-hong(College of Life Science and Technology,Xinjiang University,Urumqi Xinjiang 830046)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《中国土壤与肥料》2021年第2期270-275,共6页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金(31560032)。

摘　　要：从新疆五家渠103团盐碱地盐穗木根际的3份土样中筛选出10株产ACC脱氨酶的菌株,通过对这10株菌株的ACC脱氨酶活性和耐盐能力的测定,结果显示其中有4株菌株ACC脱氨酶活性较高,且能在盐(NaCl)浓度12%生长。根据ACC脱氨酶活性以及耐盐性能的测定结果,选择W5、W8、W9这3株菌株进行鉴定,通过生理生化实验、Biolog鉴定、16S rDNA测序分析后,鉴定结果显示W5、W8为乳酪短杆菌(Brevibacterium casei),W9为普沙根瘤菌(Rhizobium pusense)。In this paper,10 strains of ACC deaminase-producing bacteria were isolated from three soil samples from the rhizosphere soil of Halostachys caspica in saline-alkali soil of the 103 group in Wujiaqu,Xinjiang.By measuring the ACC deaminase activity and salt tolerance of these 10 strains,the results showed that 4 strains of them had higher ACC deaminase activity,and 4 strains were able to grow at 12% salt(NaCl)concentration.Strain W5,W8 and W9 with excellent salttolerance and high ACC deaminase activity were selected for identification.After physiological and biochemical experiments,Biolog identification,and 16 S rDNA sequencing,the identification results showed that W5 and W8 were Brevibacterium casei and W9 was Rhizobium pusense.

关 键 词：盐穗木 耐盐细菌 ACC脱氨酶 植物根际促生菌 菌种鉴定 

分 类 号：S793.9[农业科学—林木遗传育种]