HPLC测定益肾骨康颗粒中莫诺苷、马钱苷、野黄芩苷、柚皮苷的含量  

Determination of Morroniside,Loganin,Scutellarin and Naringin in Yishen Gukang Granules by HPLC

作　　者：宋京美[1] 龚韬[1] 杨薇 胡银燕 严文利[1] 王夏 冯利 SONG Jingmei;GONG Tao;YANG Wei;HU Yinyan;YAN Wenli;WANG Xia;FENG Li(Beijing Institute of Clinical Pharmacy,Beijing 100035,China;National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100021,China)

机构地区：[1]北京市临床药学研究所,北京100035 [2]国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院,北京100021

出　　处：《中国现代应用药学》2021年第7期841-844,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：北京市科技计划项目(Z171100001717017);中国医学科学院临床与转化基金项目(2019XK320072)。

摘　　要：目的通过HPLC对益肾骨康颗粒中莫诺苷、马钱苷、野黄芩苷、柚皮苷的含量进行定量分析。方法色谱柱为Kinetex EVO C18(150 mm×4.6 mm,5μm),进样量为10μL,流动相采用乙腈(A)和0.5%磷酸水溶液(B)进行梯度洗脱。检测波长设为240 nm(莫诺苷、马钱苷)、283 nm(柚皮苷)、335 nm(野黄芩苷)进行检测。结果莫诺苷、马钱苷、野黄芩苷、柚皮苷标准曲线分别为Y=1.8978×106X‒22664.29(r=0.9999),Y=1.7920×106X+3040.43(r=0.9999),Y=3.7459×106X+3287(r=0.9999),Y=1.8540×106X+654.57(r=0.9998);线性范围分别为0.3635~2.5445,0.2382~1.6676,0.0871~0.6094,0.0783~0.5482μg,平均加样回收率分别为98.25%,96.35%,95.74%,95.76%,RSD分别为0.85%,0.92%,1.06%,0.67%。结论该法操作简单,结果具有较好的仪器精密度、稳定性和重复性,可作为益肾骨康颗粒质量控制的有效方法,而不同批次益肾骨康颗粒有效成分含量存在不同程度差异。OBJECTIVE To determine the content of morroniside,loganin,scutellarin and naringin in Yishen Gukang granules by high performance liquid chromatography(HPLC).METHODS The HPLC method was performed on Kinetex EVO C18 column(150 mm×4.6 mm,5μm).The gradient elution was adopted with the mobile phase consisting of acetonitrile(A)and 0.5%phosphoric acid aqueous(B),and the injection volume was 10μL.The detection wavelengths were set at 240 nm(morroniside and loganin),283 nm(naringin)and 335 nm(scutellarin).RESULTS The calibration curves of morroniside,loganin,scutellarin and naringin were Y=1.8978×106X‒22664.29(r=0.9999),Y=1.7920×106X+3040.43(r=0.9999),Y=3.7459×106X+3287(r=0.9999),Y=1.8540×106X+654.57(r=0.9998)and the linear ranges were 0.3635‒2.5445,0.2382‒1.6676,0.0871‒0.6094,0.0783‒0.5482μg,respectively.The average recoveries of morroniside,loganin,scutellarin and naringin were 98.25%,96.35%,95.74%,95.76%and the RSD were 0.85%,0.92%,1.06%,0.67%,respectively.CONCLUSION The method is simple,accurate,stable and reproducible for the quality control of Yishen Gukang granules.The contents of active ingredients in different batches of Yishen Gukang granules are different.

关 键 词：益肾骨康颗粒 莫诺苷 马钱苷 野黄芩苷 柚皮苷 高效液相色谱法 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]