大麦株高类性状的遗传分析与QTL定位  

Genetic Analysis and QTL Mapping of Plant Height-related Characters in Barley

作　　者：姚佳延 于国琦 洪益 吕超[1] 许如根[1] YAO Jiayan;YU Guoqi;HONG Yi;LüChao;XU Rugen(Key Laboratory of Plant Functional Genomics of the Ministry of Education,Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Institutes of Agricultural Science and Technology Development, Yangzhou University,Yangzhou 225009,China)

机构地区：[1]植物功能基因组学教育部重点实验室,江苏省作物基因组学与分子育种重点实验室,江苏粮食作物现代产业技术协同创新中心,扬州大学农业科技发展研究院,江苏扬州225009

出　　处：《华北农学报》2021年第2期28-32,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点研发计划(2018YFD1000703,2018YFD1000700);国家大麦青稞产业技术体系建设专项(CARS-05);江苏高校优势学科建设工程项目。

摘　　要：为发掘大麦株高类性状的QTL位点,改良大麦品种株高性状。以我国饲用大麦泰兴9425与日本啤酒大麦Naso Nijo构建的177份DH群体及亲本为材料,考查2种环境下参试材料的株高、穗下节间长、穗长3个株高类性状,结合已构建的分子标记连锁图谱,采用基于完备区间作图法的Windows QTL IciMaping V4.1.0.0软件进行QTL定位分析并分析株高类性状的遗传特性。结果表明:株高、穗长、穗下节间长主要受遗传因素控制,同时受试点的生态条件和生产条件影响,3个株高类性状的遗传力分别为78.98%,89.31%,84.50%。株高与穗长、株高与穗下节间长、穗长与穗下节间长均呈极显著正相关,相关系数分别为0.688,0.862和0.600,表明株高越高其穗长、穗下节间长越长。2个试点定位到6个株高QTL、5个穗长QTL及4个穗下节间长QTL,共15个QTL,除第1,6号染色体外,其他染色体上均有分布,LOD值为3.50~32.46,对表型变异的解释率为1.53%~38.30%。其中qPH-4-1、qPH-7-2、qSL-2-1、qSL-2-2、qSIL-2-1、qSIL-3-2均未见报道,可能为新位点,为大麦株高类性状的改良与分子标记辅助育种奠定基础。In order to discover the QTL of barley plant height-related characters,improve the barley plant height-related characters.Chinese forage barley Taixing 9425 and Japanese beer barley Naso Nijo as well as 177 doubled haploid lines,generated from a cross between Taixing 9425 and Naso Nijo,were used as materials to examine the three plant traits of plant height(PH),internode length under spike(SIL),and spike length(SL)in two environments.Characters,combined with the constructed molecular marker linkage map,using Windows QTL IciMaping V4.1.0.0 software based on the complete interval mapping method for QTL mapping analysis,also the genetic characteristics were analyzed.The results showed that:plant height,spike length,and internode length under spike were mainly controlled by genetic factors,and at the same time affected by the ecological conditions and production conditions of the pilot.The heritability of the three traits was 78.98%,89.31%and 84.50%,respectively;Plant height was extremely significant positive correlation with the spike length and the internode length under the spike.Plant height and spike length,plant height and the internode length under the spike,and spike length and the internode length under the spike were all significantly positively correlated,with correlation coefficients of 0.688,0.862 and 0.600,respectively,indicating that the higher the plant height,the longer the spike length and the internode length under the spike.The 2 pilot sites mapped 6 QTL for plant height,5 QTL for spike length,and 4 QTL for internode length under spike.A total of 15 QTL are located on all chromosomes except chromosomes 1 and 6.The LOD value was 3.50-32.46,the explanation rate of phenotypic variation was 1.53%-38.30%.Among them,qPH-4-1,qPH-7-2,qSL-2-1,qSL-2-2,qSIL-2-1 and qSIL-3-2 have not been reported,and may be new loci.This lay the foundation for the improvement of barley plant height-related characters and molecular marker assisted breeding.

关 键 词：大麦 株高类性状 QTL定位 DH群体 遗传分析 

分 类 号：S512.03[农业科学—作物学] Q78[生物学—分子生物学]