反相高效液相色谱法测定参芪降糖胶囊中梓醇含量  

Content Determination of Catalpol in Shenqi Jiangtang Capsules by RP-HPLC

作　　者：冯光军 罗丹 冉凤英 杨洋 杨光义 梅全喜 陈琴华 FENG Guangjun;LUO Dan;RAN Fengying;YANG Yang;YANG Guangyi;MEI Quanxi;CHEN Qinhua(Department of Pharmacy,Shenzhen Bao′an Authentic TCM Therapy Hospital,Shenzhen,Guangdong,China 518101;Department of Medical Experimental Center,Affiliated Dongfeng Hospital of Hubei University of Medicine,Shiyan,Hubei,China 442008)

机构地区：[1]广东省深圳市宝安纯中医治疗医院药学部,广东深圳518101 [2]湖北医药学院附属东风医院医学实验中心,湖北十堰442008

出　　处：《中国药业》2021年第9期63-65,共3页China Pharmaceuticals

基　　金：广东省深圳市宝安区医疗卫生基础研究项目[2020JD491];广东省深圳市宝安纯中医治疗医院院内科研项目[BCZY2021007]。

摘　　要：目的建立测定参芪降糖胶囊中梓醇含量的反相高效液相色谱(RP-HPLC)法。方法色谱柱为Linksil HPLC Column ODS C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(2∶98,V/V),流速为0.8 m L/min,检测波长为205 nm,柱温为30℃,进样量为10μL。结果梓醇质量浓度在1~100μg/m L范围内与峰面积线性关系良好;精密度、稳定性、重复性试验结果的RSD均小于2%(n=6);平均加样回收率为103.45%,RSD为1.61%(n=6)。结论该方法分离效果和重复性均良好,测定结果准确,回收率高,可用于参芪降糖胶囊中梓醇的含量测定。Objective To establish a reverse phase high-performance liquid chromatography(RP-HPLC)method for the content determination of catalpol in Shenqi Jiangtang Capsules.Methods The chromatographic column was Linksil HPLC Column ODS C18 column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.1%phosphoric acid solution(2∶98,V/V),the flow rate was 0.8 m L/min,the detection wavelength was 205 nm,the column temperature was 30℃,and the injection volume was 10μL.Results The linear range of catalpol was 1-100μg/m L,the RSDs of precision,stability and repeatability tests were less than 2%(n=6).The average recovery of catalpol was 103.45%with RSD of 1.61%(n=6).Conclusion The method has a good separation effect and repeatability,accurate results and a high recovery rate,which can be used for the content determination of catalpol in Shenqi Jiangtang Capsules.

关 键 词：参芪降糖胶囊 梓醇 反相高效液相色谱法 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]