肥大性菌核病桑椹的转录组学分析  

Transcriptome Analysis on Mulberry with Hypertrophy Sorosis Scleroteniosis

作　　者：王瑞娴 楚渠[2] Wang Ruixian;Chu Qu(School of Modern Agriculture and Biotechnology,Ankang University,Ankang Shaanxi 725000,China;Shaanxi Key Laboratory of Sericulture,Ankang University,Ankang Shaanxi 725000,China)

机构地区：[1]安康学院现代农业与生物科技学院,陕西安康725000 [2]安康学院陕西省蚕桑重点实验室,陕西安康725000

出　　处：《蚕业科学》2020年第6期685-692,共8页ACTA SERICOLOGICA SINICA

基　　金：陕西省教育厅重点实验室科研计划项目(17JS001);安康学院校内专项(2016AYXNZX019);陕西省教育厅重点实验室科研计划项目(18JS002)。

摘　　要：桑椹肥大性菌核病是果桑中发病率最高、危害最严重的病害。本研究利用第二代高通量测序技术,对正常桑椹和感染肥大性菌核病的桑椹进行转录组测序,筛选差异基因,并与GO和KEGG数据库比对进行注释,分析感染肥大性菌核病相关基因。结果显示,测序共获得30.49 GB的数据,组装得到70788条UniGene序列,其中35714条UniGene获得注释信息,占全部UniGene的50.45%。差异基因表达分析发现,与对照组相比,感染肥大性菌核病的桑椹中有2217个基因表达上调,2724个基因表达下调。根据GO功能分类可分为生物过程、细胞组分和分子功能3大类63项功能组。根据KEGG代谢通路分析,1578个差异表达基因参与120个信号通路,其中56个基因参与植物激素信号转导,包括木葡聚糖内糖基转移/水解酶(XTH)、蛋白磷酸酶、转运蛋白、生长素应答蛋白、乙烯响应因子、脱落酸受体和多种转录因子等,36个基因参与植物与病原菌相互作用,包括蛋白激酶、钙调蛋白、爆发式氧化酶和分子伴侣等。本研究丰富了桑椹研究的转录组信息,获得了差异表达基因,为深入研究桑椹肥大性菌核病的致病机制及防治技术开发提供了理论基础。Hypertrophy sorosis scleroteniosis is the most serious mulberry disease with the highest morbidity.In this study,transcriptome of normal mulberry and mulberry with hypertrophy sorosis scleroteniosis was sequenced by the next-generation sequencing technology to screen the differential genes.Furthermore,related genes of hypertrophy sorosis sclerotiniosis were analyzed by comparing with GO and KEGG database for annotation.A total of 30.49 GB data and 70788 UniGenes were obtained after assembly,of which 35714 UniGenes were annotated,accounting for 50.45%of all UniGenes.Analysis on differentially expressed genes showed that 2217 genes were up-regulated and 2724 genes were down-regulated.According to the functional classification of GO,these 63 functional groups can be divided into three categories:biological process,cell component and molecular function.KEGG pathway analysis showed that 1578 differentially expressed genes were involved in 120 signal pathways,of which 56 genes were involved in plant hormone signal transduction,including xyloglucan endotransglucosylase/hydrolase,protein phosphatase,transporter,auxin-responsive protein,ethylene-responsive factor,abscisic acid receptor and various transcription factors.Besides,36 genes were involved in plant-pathogen interaction,including protein kinase,calmodulin protein,respiratory burst oxidase-like protein and molecular chaperones.This study enriched the transcriptome study on mulberry and obtained differentially expressed genes,which could provide a theoretical basis for further study on pathogenesis and control of mulberry hypertrophic sorosis scleroteniosis.

关 键 词：桑椹 肥大性菌核病 转录组 

分 类 号：S888.71[农业科学—特种经济动物饲养]