桑树烟煤病的病原鉴定  

Pathogenic Identification of Mulberry Sooty Mold

作　　者：柴建萍[1] 江秀均[1] 谢道燕[1] 倪婧[1] 杨振国[1] 苏振国[1] Chai Jianping;Jiang Xiujun;Xie Daoyan;Ni Jing;Yang Zhenguo;Su Zhenguo(Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Mengzi Yunnan 661101,China)

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《蚕业科学》2020年第6期693-699,共7页ACTA SERICOLOGICA SINICA

基　　金：云南省农业基础研究联合面上项目(2018FG001-070);现代农业产业技术体系建设专项(CARS-18)。

摘　　要：桑树烟煤病随着桑粉虱种群在蚕区流行为害,但桑树烟煤病病原尚不明确。根据科赫法则,应用形态学方法和ITS分子技术对桑树烟煤病病原菌进行分离、接种验证及鉴定。通过菌落形态、产孢结构特征、分生孢子类型与形成方式的描述,以及病原菌DNA序列比对分析,明确桑树烟煤病的病原菌MWx为枝孢菌属(Cladosporium)真菌,属座囊菌纲(Dothideomycetes)、煤炱目(Capnodiales)、小戴卫霉科(Davidiellaceae)。该病原菌的鉴定为桑树烟煤病、桑粉虱综合防控研究奠定了工作基础。Mulberry sooty mold along with prevalence of Pealius mori can cause severe damage in sericulture area.However,its pathogen was still unclear.Therefore,pathogen of mulberry sooty mold was isolated,and its inoculation was verified and identified in line with Koch's postulates based on morphological observation and internal transcribed spacer(ITS)identification.MWx,the pathogen of mulberry sooty mold,was identified as Cladosporium,belong to Dothideomycetes,Capnodiales,Davidiellaceae according to its colony morphology,sporulation structure,conidia type,formation mode,and DNA sequence alignment.This pathogenic identification will lay a foundation for the comprehensive control of mulberry sooty mold and Pealius mori.

关 键 词：桑树 烟煤病 病原鉴定 

分 类 号：S888.719[农业科学—特种经济动物饲养] Q939.5[农业科学—畜牧兽医]