苗药铁筷子挥发油的化学成分分析及抑制BGC-823细胞增殖研究  

Study on chemical composition of volatile oil of Miao medicine Chimonanthi Radix and its inhibitory effect on BGC-823 cells

作　　者：鲁杰 谢丹 阮婧华 范东生 查鑫 辛加敏 LU Jie;XIE Dan;RUAN Jinghua;FAN Dongsheng;ZHA Xin;XIN Jiamin(Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China)

机构地区：[1]贵州中医药大学,贵州贵阳550002 [2]贵州中医药大学第一附属医院,贵州贵阳550001

出　　处：《贵州科学》2021年第1期26-30,共5页Guizhou Science

基　　金：2017年度学术新苗培养及创新探索专项项目(黔科合平台人才〔2017〕5735号-32);贵州省2018年度科技支撑计划项目(黔科合支撑〔2018〕2821)。

摘　　要：目的:研究苗药铁筷子挥发油成分及挥发油抗肿瘤作用。方法:采用水蒸气蒸馏法提取铁筷子挥发油,利用GC-MS联用仪分析铁筷子挥发油的化学成分。CCK8法检测苗药铁筷子挥发油对BGC-823细胞增殖的抑制作用。结果:从苗药铁筷子挥发油中鉴定了33种化合物,共占挥发油的89.71%,苗药铁筷子挥发油中主要含有棕榈酸占总油量的19.99%、亚油酸占总油量的13.27%、油酸占总油量的10.99%。抗肿瘤活性表明苗药铁筷子挥发油对BGC-823细胞的增殖抑制作用,IC_(50)值为11.793μg/mL。结论:苗药铁筷子挥发油可抑制BGC-823细胞的增长。In this study we explored the chemical composition and antitumor activity of the volatile oil of Chimonanthi Radix.The volatile oil of Chimonanthi Radix was extracted by steam distillation,and the chemical composition of the volatile oil was analyzed by GC-MS.CCK8 method was adopted to analyze the inhibitory effect of the volatile oil on the proliferation of BGC-823 cells.As a result,33 compounds were identified in the volatile oil of Chimonanthi Radix,accounting for 89.71%of the volatile oil.The volatile oil of Chimonanthi Radix mainly contained palmitic acid(19.99%),linoleic acid(13.27%),and oleic acid(10.99%).Antitumor activity tests indicated that the volatile oil of Chimonanthi Radix had certain inhibitory effect on the proliferation of BGC-823 cells,with the IC_(50) value of 11.793μg/mL.In conclusion,the volatile oil of Chimonanthi Radix could inhibit the growth of BGC-823 cells.

关 键 词：苗药铁筷子 挥发油 GC-MS BGC-823 抗肿瘤活性 

分 类 号：R932.2[医药卫生—生药学]