水稻抗稻瘟病基因Bsr-d1的SNP区域在地方品种中的变异分析  

SNP Fragment Variations Analysis of Resistant Gene Bsr-d1 to Rice Blast in Rice Landraces

作　　者：刘畅媛 孙一丁[2] 马继琼[2] 杨奕[2] 汤翠凤[2] 阿新祥[2] 郭怡卿[1] 许明辉[2] Liu Changyuan;Sun Yiding;Ma Jiqiong;Yang Yi;Tang Cuifeng;A Xinxiang;Guo Yiqing;Xu Minghui(College of Plant Protection,Yunnan Agricultural University,Kunming,650201;Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture and Rural Affairs of the People's Republicof China,Key Laboratory of Biotechnology Research of Yunnan Province,Institute of Biotechnology and Genetic Resources,Yunnan Academy of Agricultural Sciences,Kunming,650205)

机构地区：[1]云南农业大学植保学院,昆明650201 [2]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业农村部西南作物基因资源与种质创制重点实验室,昆明650205

出　　处：《分子植物育种》2021年第7期2097-2102,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2016YFD0100101)资助。

摘　　要：为揭示地方水稻品种中稻瘟病持久抗性基因Bsr-d1序列变异类型,本研究以收集到的缅甸、老挝和越南共79份地方水稻品种为供试材料,设计抗稻瘟病Bsr-d1基因特异引物对Bsr-d1基因启动子功能位点区段进行PCR扩增及测序分析。研究表明,79份地方水稻均持有抗稻瘟病Bsr-d1或bsr-d1等位基因,品种间存在18个变异位点和22个碱基的差异,根据差异所有品种可归为6种单倍型,其中H4和H6为优势单倍型,分别占测试品种的20.25%、58.23%;6种单倍型中仅H3单倍型携带抗性位点,有8个材料归为此类型,占10.13%。缅甸地方品种拥有H1(15.79%)、H2(2.63%)、H3(13.16%)、H4(18.42%)、H6(50%)等5种单倍型,优势单倍型为H6;老挝地方品种有H1(2.56%)、H3(7.69%)、H4(23.08%)、H5(2.56%)、H6(64.10%)5种单倍型,H6为优势单倍型,表明缅甸、老挝间在单倍型种类和抗性品种比例上均存在一定差异。本研究结果表明缅甸、老挝水稻资源携带持久抗性基因Bsr-d1的品种比例远高于中国品种,对中国选育稻瘟病持久抗性品种具有重要的利用价值,应加强对该地区种质的引进和分析评价。In order to reveal the nucleotide variations of the rice blast resistance gene Bsr-d1 in rice landraces from Myanmar and Laos. A total of 79 accessions of rice landraces from Myanmar and Laos were used as materials to design specific primers for rice blast resistance gene Bsr-d1 to rice blast disease for PCR amplification and sequencing analysis of the promoter region functional site segment of Bsr-d1. The results showed that 79 accessions of rice landraces from Myanmar and Laos all contained Bsr-d1 gene or bsr-d1 allele, and there were 22 variation bases of the 18 SNP locus in the varieties. According to the difference, all the varieties could be classified into 6 haplotypes, of which H4 and H6 were dominant haplotypes, accounting for 20.25% and 58.23% of the tested varieties. Only H3 haplotype carried the resistant locus in the 6 haplotypes, and 8 landraces belongs to H3,accounting for 10.13%. In Myanmar, the landraces varieties occurrenced 5 DNA haplotypes, such as H1(15.79%), H2(2.63%), H3(13.16%), H4(18.42%) and H6(50%), and the dominant haplotypes was H6. In Laos, the landraces varieties occurrenced 5 DNA haplotypes such as H1(2.56%), H3(7.69%), H4(23.08%), H5(2.56%), H6(64.10%), and the dominant haplotypes was H6. H6 is the dominant haplotype, indicating that there are some differences in haplotype types and resistant varieties proportion between Myanmar and Laos. The results showed that the proportion of rice resource contained Bsr-d1 gene in Myanmar and Laos outclass China. The germplasm has important using value for breeding rice blast lasting resistance varieties in China. It is suggested that the introduction and analysis evaluation of germplasm in this area should be strengthed.

关 键 词：水稻 种质资源 稻瘟病 抗性基因 单倍型 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]