黄瓜CsamiR156a及其靶基因CsSPLs的生物信息学分析  

Bioinformatics Analysis of CsamiR156a and Its Target Gene CsSPLs in Cucumber

作　　者：李尧尧 常怀成 周海霞[2] 吴小波[2] 李庆飞 袁敬平 李芳霞[2] 孙涌栋 Li Yaoyao;Chang Huaicheng;Zhou Haixia;Wu Xiaobo;Li Qingfei;Yuan Jingping;Li Fangxia;Sun Yongdong(Henan Province Engineering Research Center of Horticultural Plant Research Utilization and Germplasm Enhancement,College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,453003;Zhengzhou Vegetable Research Institute,Zhengzhou,450000)

机构地区：[1]河南科技学院园艺园林学院,河南省园艺植物资源利用与种质创新工程研究中心,新乡453003 [2]郑州市蔬菜研究所,郑州450000

出　　处：《分子植物育种》2021年第7期2169-2176,共8页Molecular Plant Breeding

基　　金：河南省重点研发与推广专项(202102110040;212102110130);新乡市重点科技攻关(GG2019013);河南科技学院大学生“百农英才”创新项目(BNYC2018-2-40)共同资助。

摘　　要：miR156是植物中广泛存在的一类miRNA,在逆境胁迫和植物生长发育过程中发挥了重要作用。为了深入研究CsamiR156a在黄瓜果实膨大中的功能,本研究采用生物信息学方法对CsamiR156a及其靶基因Cs SPLs(SQUAMOSA promoter-binding protein-like)进行分析。结果表明,CsamiR156a前体序列具有完整的茎环结构,成熟序列高度保守。CsamiR156a有11条靶基因,编码8个不同的CsSPLs蛋白序列。8个CsSPLs蛋白均属不稳定蛋白,多数为碱性蛋白、亲水性蛋白。二级结构均为混合型,无信号肽和跨膜结构,均为可溶性蛋白。8个CsSPLs蛋白大部分都含有糖基化位点,有17~83个不等的磷酸化位点,均定位于细胞核。系统进化树表明,黄瓜CsSPLs蛋白与甜瓜、中国南瓜和印度南瓜等物种SPLs蛋白遗传距离较近;蛋白序列分析发现,各物种SPLs蛋白均含有SBP(Squamosa promoter binding protein)功能结构域,且其位置和数量大体相同,说明SPLs蛋白功能保守。据此推测,CsamiR156a通过靶向调控Cs SPLs基因表达参与了黄瓜果实膨大。该研究结果为今后深入研究黄瓜果实膨大的分子机理提供了一定的科学依据。miR156 is widely found in plants, which plays an important role in the stress of adversity and the growth and development of plants. In order to deeply understand the function of CsamiR156 a on cucumber fruit expansion, the bioinformatics analysis of CsamiR156 a and its target genes Cs SPLs(SQUAMOSA promoter-binding protein-like) were carried out. The results showed that the precursors of CsamiR156 a had complete stem-loop and CsamiR156 a was conserved. CsamiR156 a had 11 target genes which encoded 8 different CsSPLs protein sequences.Bioinformatics analysis showed that all 8 CsSPLs proteins were unstable, and most of them were basic and hydrophilic proteins. The secondary structure of CsSPLs were mixed type. All 8 CsSPLs proteins were soluble proteins without signal peptide and transmembrane structure. Most of the eight CsSPLs proteins contained glycosylation sites and 17~83 phosphorylation sites, which might be located in the nucleus. Phylogenetic tree showed that cucumber CsSPLs shared highly similarity with SPLs from Cucumis melo, Cucurbita moschata and Cucurbita maxima. The sequence and SBP(Squamosa promoter binding protein) domain of SPLs protein among differential species were highly conserved based on the analysis of sequence alignment and conserved domain,which indicated the conserved function among differential species. It was suggested that CsamiR156 a might involve in fruit expansion of cucumber by regulating the expression of CsSPLs. These results could provide a theoretical basis for further study on molecular mechanism of fruit expansion in cucumber.

关 键 词：黄瓜(Cucumis sativus) 果实膨大 miR156a SPLs 生物信息学分析 

分 类 号：S642.2[农业科学—蔬菜学]